DNA Purification from Gels

1.Remove gel slice contain DNA fragment and place in 10 volumes of:

300 mM NaOAc,pH 7.0 300 ml 1 M NaOAC,pH 7.0

1 mM EDTA 2 ml 500 mM EDTA,pH 8.0

698 ml ddH₂0

2.Incubate at 22℃ for 30 min.Transfer gel slice to a fresh tube.

3.Place tube in a Dry Ice/Ethanol bath for 5 min.

4.Puncture the bottom of a 0.5 ml microcentrifuge tube with a needle.Place the gel slice into this tube.Place this tube inside a 1.5 ml microcentrifuge tube.

5.Centrifuge for 15 min.

6.Collect the Eluent from the 1.5 ml eppendorf tube.Extract and precipitate the DNA.