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Hi.- I have a problem with amplification of DNA by PCR. The extraction of DNA is direct from plant. i use PVP for keep out of DNA Polyphenols from the samples. The problem is: i can't view apmlificati ...

本方法通过SDS裂解细胞，蛋白酶降解蛋白，CTAB去除多糖成分 一:仪器:同方法一 二:试剂: TE、TAE缓冲液;10%SDS;5MNaCL; 20mg/ml蛋白酶K;CTAB/NaCL溶液(10% CTAB，0.7M NaCL 4.1gNaCL 溶于80ml水中，缓慢加入10gCTAB，加热溶解);25/24/1，酚/氯仿/异戊醇; 24/1，氯仿/异戊醇;异丙醇;70%及100 ...

Preparation of Silica 1. Suspend 5 g of silica (Sigma S-5631) in 50 ml of PBS. 2. Allow the silica to settle for 2h. 3. Discard the supernatant containing fine particulate matter. 4. Repeat steps 2 an ...

Sugden labMcArdle Laboratory for Cancer Research University of Wisconsin-Madison Medical School http://mcardle.oncology.wisc.edu/sugden/Protocols/html files/Filter Binding Assay.htm Last Modified 5.2. ...

(adapted from Bruce A. Roe Department of Chemistry and Biochemistry The University of Oklahoma Norman Oklahoma 73019 broe@ou.edu) A. Sonication The generation of DNA fragments by sonication is perfor ...

Does anybody know how to isolate single-stranded DNA from mammalian cells? Thanks for responding. Yin -- -------------------------------------------------------------------------------- Hello I don't ...

An U ...

1.Remove gel slice contain DNA fragment and place in 10 volumes of: 300 mM NaOAcpH 7.0 300 ml 1 M NaOACpH 7.0 1 mM EDTA 2 ml 500 mM EDTApH 8.0 698 ml ddH20 2.Incubate at 22℃ for 30 min.Transfer gel sl ...

Non-radioactive Probes I. Via random hexamers 1. Solutions: 10X hexa nt miundefined: 500 mM Tris-Cl pH 7.2 100 mM MgCl2 1 mM dithioerythritol (DTE) 2 mg/ml BSA 62.5 A260 units/ml (1.56 mg/ml) random hexan ...

Solutions Protocol: Digest 5-10 μg genomic DNA overnight with restriction enzyme of choice. Run digested gDNA on 0.8% TAE gel with marker (with no ethidium bromide). Transfer Setup: 1. Remove gel ...

Steven FinkbeinerDepartments of Neurology and PhysiologyUCSF http://gweb1.ucsf.edu/labs/finkbeiner/Protocol/protocol_list/cloning.shtml DIGEST: 1.Digest 10 - 20μg DNA to isolate either the desired ...

DNA sequencing is the determination of the precise sequence of nucleotides in a sample of DNA. The most popular method for doing this is called the dideoxy method. The equipment and supplies:·S ...

Recovery of DNA from LMP (Low Melting Point) Agarose Gel 1. Separate DNA fragments through an LMP agarose gel containing ethidium bromide (0.5 microgram/ml). 2. Detect DNA by irradiating the gel with ...

Alkaline lysis miniprep 1. Grow bacteria overnight in 37℃shaking incubator with lids very loose and taped on. I normally use 5 ml of liquid medium in a 50ml conical bottom tube. Be sure to include the ...

This protocol was written by Jean-Pierre Issabased on Adams et al.* Kam-Wing Jair has made some useful shortcuts that work well if you are careful.Here is .This assay can be used to measure activity i ...

(adapted from Bruce A.RoeDepartment of Chemistry and BiochemistryThe University of OklahomaNormanOklahoma 73019 broe@ou.edu) A.Large scale double-stranded DNA isolation The method used for the isolati ...

What are the differences between phasmidcosmidsphagesand plasmids. -- -------------------------------------------------------------------------------- Hope this brief explanation helps. Phasmid are hy ...

Cepko/Tabin Lab Harvard University This protocol works well from a 5 ml starting culture or a 1 ml starting culture (adjust volume accordingly). http://axon.med.harvard.edu/~cepko/protocol/mike/S2.ht ...

Abstract: The protocol is simple and fairly rapid.It does not require the use of organic solvents but rather utilizes salt extraction to precipitate contaminating proteins.High quality DNA is obtained ...

Donis-Keller Lab Manual Department of Genetics in Washington University School of Medicine http://hg.wustl.edu/hdk_lab_manual/plasmid/plsmid13.html Purpose: To maintain lab stock of highly efficient ...