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DNA PrepPrepare DNA via your favorite method. You may find a protocol underMini Yeast Genomic Prep.Restriction Digest1.Digest DNA with appropriate restriction enzyme. Digest 10μg of Yeastchromosoma ...

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Hi Can you tell me what do "SSC" in a southern blot how he act on DNA or in the protocole. (excuse-me for my traduction i never write in english) -Sebela- ----------------------------------- ...

Transgenic Mouse and Gene Targeting Facility Tg 008 Southern Blotting Materials and Equipment Hybaid hybridization oven (ISC BioExpressH-9250) Vacuum oven (VWR52201-218) Ludlum Geiger Counter Radiati ...

Hey I am analyzing the methylation status of two regions: one ecompasses 500bp the other is around 800bp. Could I just designed primers at flanking sites and amply these two regions? I found usually o ...

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Jacks Lab Center for cancer researchMIT 1. Each tail should be in a clean eppendorf tube. 2. Add 500µl of tail lysis buffer containing Proteinase K (PK) to each tube. 3. Incubate tai ...

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DNA Fragment Purification from Agarose or Acrylamide For fragments from 200 bp to 10 kb the agarose purification is ideal.For smaller fragments (20 bp to 400 bp)the acrylamide purification is preferre ...

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Purpose: The method is used to clone smaller portions of inserts (up to 15 kb in size) which previously have been cloned in YACs phage cosmids or other plasmids. Time required: &nbs ...

HiI'm starting to work whit 5' cytidine on plants. I want to know if it's possible to prepare a stock solution to store it at -20C. In that conditions the drug lose the desmetilant capacity?I've wrote ...

1.grow plants in trays of 96 and leave two spots open (for the PCR controls) 2.harvest 1 to 2 young and green leaves (1cm2 /plant at rosette stage if possible). Use 96 well plates (1 or 2ml E&K p ...

Saghai-Maroof MA Soliman KM Jorgensen RA & Allard RW (1984) PNAS 81:8014-8018 DNA successfully isolated from fungal species of Cochliobolus Aternaria and Fusarium. The key elements in this prep ar ...

Pick colonies into 0.5ml of SD-Leu (or other appropriate SD medium) Vortex for 1min Leave to grow O/N for 18-24h at 30℃ 230-250rpm (best in 5ml bijou) Spin yeast culture at 13000rpm for 5 min (microfu ...

i want to know why exactly tris is used as a buffer when we can use any inorganic salt also to reach pH 8 so what is the function of tris? ------------------------------------------------------------ ...

Steve Hahn Last Modified FriApr 252003 Wear gloves throughoutuse RNAse free solutions (either autoclaved or sterile filtered)and clean bench and pipetmen with 95% ethanol before use to eliminate any s ...

DNA Transfer1. Depurination of DNA fragments: Wash gel in 0.25 M HCl 5' (small gels) 7' (large gels) 2. Denaturation: Wash gel in (0.5 M NaOH 1.5 M NaCl) for 20' (small) to 30' (large). 3. Neutralizat ...