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表达分析、分子生物学研究以及新的分析软件工具将会推进生物系统水平的研究。 文/EricChan西雅图RosettaBiosoftware数据分析师译/李亚萍 在基因表达研究中，广泛的基因分析可以对生理状态或者是一个细胞表型有关的基因进行系统监测。可以利用高通量分析在数据输出和获取数据快捷两方面的优势，对药物发现过程中的药靶候选基因进行鉴定，在假设驱动的研究中，该技术也提供了必须的系统背景知识。一旦 ...

Contributor: Suprya Jayadev Date: April 111996 1)Weigh out the appropriate amount of DEAE sephadex and place in a 15 ml conical tube. 2)Wash resin three to four times with zenopure water.Mix gel with ...

三级结构主要针对球状蛋白质而言的 是指整条多肽链由二级结构元件构建成的总三维结构，包括一级结构中相距远的肽段之间的几何相互关系骨架和侧链在内的所有原子的空间排列。在球状蛋白质中，侧链基团的定位是根据它们的极性安排的。蛋白质特定的空间构象是由氢键、离子键、偶极与偶极间的相互作用、疏水作用等作用力维持的，疏水作用是主要的作用力。有些蛋白质还涉及到二硫键。 如果蛋白质分子仅由一条多肽链组成，三级结构就是 ...

This procedure is used to break cells that contain a protein to be purified.This procedure is valid only for the use on Appling's lab sonicatior. PREPARATION 1.Place your sample in a 15ml elongated p ...

Fusion Protein Isolation 融合蛋白分离纯化 Peter Novick LabDepartment of Cell Biology Yale University School of Medicine http://info.med.yale.edu/cellbio/Novick/Second/Protocols/Fusion.pdf 1.Start a 5 ml overn ...

1x 40min - overnight 50% MeOH 12% Acetic Acid 1x 30min ...

Summary WCE Extracts are prepared from strain SHY282 (TFC4-Flag-EE (HpaI)).TFIIIC complex is first purified by flag affinity: TFC4 contains a single copy of the flag epitope.The flag purified TFIIIC i ...

Reagents: ChIP sonication Buffer (1% Triton X-1000.1% Deoxycholate50 mM Tris 8.1150 mM NaCl5 mM EDTA): 10 ml 10% Triton X-100 1 ml 10% Deoxycholate 5 ml 1 M Tris-Cl pH 8.1 1 ml 0.5 M EDTA 3 ml 5 ...

Lysis buffer. The choice of lysis buffer depends on what the cells were labeled with and whether you want to obtain an active kinase.The lowest background is obtained with RIPA buffer without EDTA.EDT ...

蛋白质立体结构原则 1.由于C=O双键中的π电子云与N原子上的未共用电子对发生“电子共振”，使肽键具有部 分双键的性质，不能自由旋转。 2.与肽键相连的六个原子构成刚性平面结构，称为肽单元或肽键平面。但由于α-碳原子与其他原子之间均形成单键，因此两相邻的肽键平面可以作相对旋转。此单键的旋转决定两个肽键平面的位置关系，于是肽键平面成为肽链盘曲折叠的基本单位 ...

Bradford Protein Assay- a microassay for determining protein content in a 96-well microtiter plate format Laboratory of P.J.HansenDept.of Animal SciencesUniversity of Florida http://www.animal.ufl.edu ...

This is a protocol from Engelke et.al.(1990)which was modified by Dr.Baron.The enzyme has been used for RT-PCRgenotyping and cloning. Solutions 1000X IPTG 0.5M IPTG 1.19g IPTG up to 10 ml wih sterile ...

常用核酸和氨基酸代码 Amino Acids Table Here is a list of the standard one-letter amino acid codes and their three-letter equivalents. The synonymous codons and their depiction in the IUB codes are shown. You sh ...

Hahn Lab (adapted from J.LeatherwoodPtashne lab) last modified MonAug 272001 Cell Growth 3 liters of cells are grown in YPD (3% glucose)to A600 of 3-5.Antifoam A (two drops from a P200 pipetman)is ad ...

Procedure A: Preparation of the cell lysate 1.Rinse a 60 mm culture dish of confluent cells with 10 mM Tris-HClPH 7.40.15 M NaCl1 mM MnCl2 and 0.2 mM PMSF. 2.Lyse the cells with 0.5ml cold buffer (1 ...

Before trying this methodtry rapid protein prep which involves boiling yeast in SDS PAGE buffer 1.Grow 100 ml yeast to A600 ~1.0.If the yeast are overgrownuse proportionally less cells for the prep. 2 ...

Use Promega's nuclease-treated -Met (L4210)or -Cys (L4240).The lysate is the samejust the amino acid mixes differ.The Promega protocols work fine as followsalthough it is often best to scale them down ...

Purpse The prtcl describes hw t prepare human tnsil lysate fr use in purificatin f ICAM-1LFA-1and PNAd. Materials Safety Equipments Lab Cat Latex Glves Face Mask Bench Paper Fresh human tnsil tissue ...

Contributor: Suprya Jayadev Date: September 211993 Resin preparation: 1)Measure out appropriate volume of resin into a 50 ml conical tube. --> NOTE: 1 ml of DEAE-Sephacel should bind approximately ...

Hattoti's protocol adapted to cell culture : Hattori MTugores AVeloz LKarin MBrenner D (1990)A simplified method for the preparation of transcrip-tionally active liver nuclear extracts.DNA Cell Biol.V ...