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A．Materials 1.NUNC-Immuno Plate IIF. 2.Horseradish Peroxidase-Streptavidin (Zymed; 43-4323) 3.PBS or Balanced Salt Solution (BSS). Do not use RPMI or any medium that contains bioti ...

The indirect ELISA is used primarily to determine the strength and/or amount of antibody response in a sample whether it is from the serum of an immunized animal or the cell supernatant from growing h ...

A. Reagents 1. DMEM:10% FBS 2.DMEM:10% FBS:1% HEPES (DFH) 3.10X Substrate Buffer pH 6.0 36.6 gCitric Acid monohydrate113.5 gPotassium dibasic phosphateDissolve in 900 ml ...

1. Reagents Sodium Borohydride (NaBH4) - 1 mg/mlCarbonate:Bicarbonate Buffer pH 9.6 Sodium Periodate (NaIO4) - 1 mM 2.14 mg/10 ml Acetate Buffer 10mM Acetate Buffer pH 4.50.82 g sodium acetate in 90 m ...

A. Reagents PBS PBS:0.1% BSA:0.05% Tween 20 (PBT) PBS:2% BSA 10X Substrate Buffer pH 6.036.6 g Citric Acid monoh ...

多肽药物在治疗上的重要性越来越引起广大药学工作者的重视。根据肽链的构成可将多肽分为同聚肽(Homomeric)和杂聚肽(Heteromeric)两大类前者完全由氨基酸组成后者是由氨基酸部分和非氨基酸部分组成的如糖肽。根据肽键的结构又分为直链肽和环肽。其中直链肽的研究最为广泛和深入尤其在直链肽的合成技术方面无论是液相法还是固相法都已成熟。虽然许多直链肽体外具有很好的生物活性和稳定性但是进入体内后活性 ...

Agarose gel electrophoresis (2) is employed to check the progression of a restriction enzyme digestion to quickly determine the yield and purity of a DNA isolation or PCR reaction and to size fraction ...

Q：SDS－PAGE电泳的基本原理？ A：SDS-聚丙烯酰胺凝胶电泳，是在聚丙烯酰胺凝胶系统中引进SDS（十二烷基硫酸钠），SDS能断裂分子内和分子间氢键，破坏蛋白质的二级和三级结构，强还原剂能使半胱氨酸之间的二硫键断裂，蛋白质在一定浓度的含有强还原剂的SDS溶液中，与SDS分子按比例结合，形成带负电荷的SDS-蛋白质复合物，这种复合物由于结合大量的SDS，使蛋白质丧失了原有的电荷状态形成仅保持原 ...

1. Rinse gel on glass plate briefly with distilled water. 2. Wash gel in fresh 50% ETOH-12% HAc with shaking 3 times 1 hour each. (Fix IEF gel ON in 10% TCA). 3. Wash gel four times with 10% ETOH-5% H ...

薄层色谱法作为一种快速简便的色谱技术已在中草药分析、毒物分析等众多领域中广泛应用。在传统薄层色谱法中，展开剂依靠毛细作用通过薄层吸附剂来完成对组分的分离，因此其分离时间不可控，而且随着展开距离的增加，溶剂前沿的移动逐渐减慢，被分离组分的扩散也越来越严重。针对此情况，分析学家对薄层系统进行了改进，发展了薄层色谱的一个重要分支强迫流动薄层色谱(FFPC)。FFPC是通过外力强迫展开剂在吸附剂中运动，它 ...

METHOD for Western Blots: 1.While your SDS-PAGE gel is runningmake your transfer buffer and chill to 4℃.Check that you have a frozen buffer dam is ready (stored in freezer next to Shikhatop shelf to t ...

DunnAnal.Biochem.1986: 157 GeorgiaTimes"1.5L GeorgiaTimes"1.0L GeorgiaTimes"10mM NaHCO3 ...

ECL or autoradiography? ECL is an appealing technique because it is quick and very sensitive and does not expose the investigator to radioactivity.The use of radioiodinated protein A to detect bound a ...

薄层色谱，或称薄层层析（thin—layer chromatography），是以涂布于支持板上的支持物作为固定相，以合适的溶剂为流动相，对混合样品进行分离、鉴定和定量的一种层析分离技术。这是一种快速分离诸如脂肪酸、类固醇、氨基酸、核苷酸、生物碱及其他多种物质的特别有效的层析方法，从50年代发展起来至今，仍被广泛采用。 一、基本原理 薄层层析是把支持物均匀涂布于支持板（常用玻璃板，也可 ...

Materials •Suspension culture of fibroblast cells (1 liter) •35 mM Tris-HClpH 7.4140 mM NaCl (TBS buffer) •10 mM Tris-HClpH 7.510 mM KCland 1.5 mM magnesium acetate (TBS-M) •10X T ...

Motor proteins of several kinesin family groups have now been crystallized: monomeric Kinesin-1 motor domains from humanrat and Neurospora (Kull et al.1996; Sack et al.1997; Song et al.2001)and dimeri ...

Materials Milipore filter type HA 0.45 micron Culture plates (Linbro model 76-033-05) Protein in DDW or HEPES buffer (10 mg/ml) Vacuum grease Syringe with 18 G needle Procedure 1.Millipore filter a ...

1.Fix gel in 50% methanol for greater than 1 hour rinse gel in dH2O 3 times 5 minutes each place back in 50% methanol for 1 hour. 2.Stain gel in 100 mls of stain solution for 30 minutes 3.Rinse gel i ...

SDS-PAGE: gel electrophoresis of proteinsTECHNIQUE is to set up gel plates before you mix the gel mixes.Use the THIN spacers and choose a comb--number of wells varies.Make both resolving gel and stack ...

1.Rinse gel on glass plate briefly with distilled water. 2.Wash gel in fresh 50% ETOH-12% HAcwith shaking3 times1 hour each.(Fix IEF gel ON in 10% TCA). 3.Wash gel four times with 10% ETOH-5% HAc for ...