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Rapid elution of DNA from agarose gels James Movius Hahn LabLast modified Sun Nov 1 1998A method of quickly purifying agarose gel DNA fragments for use in subsequent reactions such as further restrict ...

OverviewProcedure 1. Preheat the CTAB Isolation Buffer at 60℃.2. Grind 2 g of fresh leaf tissue to a powder in Liquid Nitrogen in a chilled mortar and pestle.3. Scrape the powder into a chilled 50 ml ...

Kitto Lab The University of Texas at Austin http://research.cm.utexas.edu/bkitto/Kittolabpage/Protocols/Microbiology/electroporation.html This procedure prepares glycerol stock cultures of bacteria fo ...

Preparation of plasmid DNA : a modified mini alkaline-lysis/PEG precipitation procedure. ABI; 1995MaterialsGTE buffer (50mM glucose 25mM Tris-HCl (pH8.0) 10mM EDTA (pH 8.0)) (200µl/tube) 0.2N NaOH / 1 ...

1. Pour a vertical acrylamide gel using TEA buffer. A 4 % non denaturing gel is correct for most applications.2. Run out DNA fragments. For fragments greater than 500 bp run the xylene cyanol dye to t ...

Hidoes anyone know what is the best temperature and time for Isoprop precipitation of DNA? I usually do at room temperature and centrifuge immediately. Can I improve this? And would it ma ...

CTAB TECHNIQUE / Method / Schedule / Protocol (JPB) FOR DNA ISOLATION / DNA EXTRACTION FROM PLANT LEAF / LEAVES SAMPLES(see also if both DNA and RNA are needed)Reagents neededCTAB buffer2% CTAB 20gm C ...

1) Digest DNA with two restriction enzymes such that one end is ExoIII susceptible (blunt or 5' overhang) and the other is ExoIII resistant (5' recessed). The ExoIII susceptible end should be such tha ...

NOTE: THIS IS FINE FOR SOUTHERNS BUT NOT FOR SCREENING BY PCR. (from Ruixia 7/99 from protocol by Stef Oehen 7/94). 1. Put 1 cm tail in 1.5 ml microcentrifuge tube.2. Add 500µl Tail Buffer and 30 µl P ...

Phenol-based Method for the Isolation of DNA Fragments from Low-Melting Temperature AgaroseReference:Favre D. 1992. Biotechniques vol. 13 1.Cut out slice containing DNA smallest size possible. 2.Estim ...

1. Pick single colony and inoculate 5 ml of LB broth containing 200 g/l ampicillin or 1mg/5ml. Optional: Use a 15ml conical tube with a loosened cap and a piece of tape to hold it in place. Shake at 2 ...

Used to removed unincorporated nucleotides from labelling reactions.Prepare Sephadex G-50 (medium) by adding appropriate amount of dry beads to 100 ml TE buffer such that the beads will swell to 50 ml ...

The following guidelines should be taken into account when designing modified oligonucleotides. 1.Sequence Length - SYNTHEGEN can synthesize oligonucleotides from 5 to 110 bases in length. Most sequen ...

CAT ASSAY 1. Transfer cells to a 15 ml tube. 2. Add 5 ml TBS- to flasks shake & pour into tubes. 3. Spin down the cells @ 1k rpm for 5'. 4. Resuspend in 1 ml TBS- . 5. Transfer to 1.5 ml tubes. 6. Spi ...

Author: Long-Cheng Li Source: Protocol Online Abstract: Simplified method for preparing G A ladder run along with footprinting reaction. It's much simple than the original Maxima-Gilbert sequencing re ...

Protocol for extracting DNA from ES Cells starting from the 96-well plate but processing in an eppendorf tube to recover more of the DNA . NOTE- THIS TAKES A LOT OF TIME if you do the whole plate this ...

Diatomaceous Earth-based Midi-prep Note: This procedure is the method of choice for isolating double stranded plasmid-based templates for the Sequenase Dye-Labeled Terminator Sequencing Reactions. A. ...

Important : Extract the DNA within one week of receiving samples. Samples that have been processed should be frozen to prevent degradation. Freeze samples between use each day.1. Add 600 m l of 50 mM ...

1. Remove gel slice contain DNA fragment and place in 10 volumes of:300 mM NaOAc pH 7.0 300 ml 1 M NaOAC pH 7.01 mM EDTA 2 ml 500 mM EDTA pH 8.0698 ml ddH2O2. Incubate at 22 ℃ for 30 min. Transfer gel ...

CTAB TECHNIQUE / Method / Schedule / Protocol (JPB) FOR DNA ISOLATION / DNA EXTRACTION FROM PLANT LEAF / LEAVES SAMPLES(see also DNA RNA double isolation procedure if both DNA and RNA are needed)Reage ...