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RNA extraction buffer:0.1M NaCl2% SDS50mM Tris/HCl (pH9)10mM EDTA20mM %26szlig;-mercaptoethanol1. Grind leaf tissue on liquid N in mortar and pestle.2. Transfer the ground tissue to a 10ml conical bot ...

Steve Hahn. last modified Sat Oct 17 1998Mix the following in an 0.5 ml eppendorf tube:10-20 micrograms total yeast RNA10 microliters hybridization mixH2O to bring the final volume to 25 microliters40 ...

人、小鼠和大鼠的多种组织来源的高质量mRNA经电泳分离后预转于尼龙膜上预制的即用型Northern杂交膜，免去RNA电泳操作过程可检测范围：0.5-10kb可重复使用提供ExpressHybTM快速杂交液样品可靠的优良品质从1991年始，公司就向广大的生命科学研究者提供高质量的预制杂交膜。而MTN™膜更是在国际著名的学术期刊上有将近3000篇文献引用。严谨的制作过程1.由人、小鼠和大鼠的组织经Ol ...

siRNA Database Searchable database of Silencer ™ Validated and Pre-designed siRNAs to 34000 human mouse and rat targets. All siRNAs in the database have been designed for maximum potency and specifici ...

The use of small interfering RNAs (siRNAs) to induce targeted gene silencing in mammalian cells offers researchers a powerful tool for analyzing gene function. Ideally one would like to work with indi ...

In vitro transcription with yeast nuclear extract Steve HahnWear gloves throughout use RNAse free solutions (either autoclaved or sterile filtered) and clean bench and pipetmen with 95% ethanol before ...

N.B: Gloves should be worn at all times during preparation of in vitro RNA. All solutions should be RNase-free i.e. made with DEPC water if home-made or bought specifically to use for RNA work. You wi ...

Run-on transcription monitors the regulation of transcription in isolated nuclei.A. Preparation of Nuclei - (do everything at 0℃ to 4℃ in 50 ml tubes)1. Pellet between 30 and 300 million cells at 1500 ...

Polymerase III in vitro Transcription Steve HahnFor the following reactions use appropriate shielding and dispose of radioactive waste properly!A 20 microliter transcription reaction contains:4.0 μ ...

1 ml linearized DNA template pBS-SK- ERD (X-E) (0.1-0.5 mg)7.8 ml DDW5 ml 5X transcription buffer ( stratagene )1 ml 750 mM DTT1 ml Rnasin (RNase inhibitor; 15U/ml)1 ml 10 mM ATP (pH 7)1 ml 10 mM CTP ...

The following protocol is for MEGAscript II Kit (Ambion). 1. PCR amplify the DNA template. The 5'-end of the template should contain the minimum promoter sequenences of T7 or Sp6 or T3.A 5-primer with ...

Transcription of cRNA probeReagents/SolutionsDNA (PCR or plasmid) with bacteriophage RNA polymerase promoter in suitable orientation (see note 1 ) ~1µg/µl in TE buffer 10mM MgCl2 5x Transcription Buff ...

T℃OS-M6 cells grow in 6-well plates 2ml media total T℃V1PD cells grow in 100mm plates.1: Aspirate off the media from the cells and was the cell monolayers with 2x5ml (To 100mm dish) or 2x1ml (to 6-wel ...

Based on: Wan C.-Y. and Wilkins T.A. 1994. Anal. Bioch. 223:7-12. 1.Collect young expanding leaves (or other tissue) and freeze in liquid N2 and store at -80℃. 2.Pulverize tissue to a fine powder in a ...

RNA extraction buffer:0.1M NaCl2% SDS50mM Tris/HCl (pH9)10mM EDTA20mM ß-mercaptoethanol1. Grind leaf tissue on liquid N in mortar and pestle.2. Transfer the ground tissue to a 10ml conical bottom cent ...

RNA interference (RNAi ) by double stranded RNA (dsRNAs) molecules of approximately 20-25 nucleotides termed short interfering (siRNAs) is a powerful method for preventing the expression of a particul ...

Chemicals needed:Glucose L-( )-Arabinose Sigma Cat # A3256 L-Rhamnose Sigma cat # R3875 Cb (carbenicillin) Sigma cat # 1389DL-p-Chlorophenylalanine Sigma cat # C6506Media Recipes:YEG recombination pla ...

RNA interference (RNAi) is a biological process in which the introduction of double-stranded RNA (dsRNA) into a cell results in targeted post-transcriptional gene silencing.Historically RNAi has been ...

We use the Promega Ribomax Large Scale RNA Production System T7 (Cat. No.: P1300) to produce dsRNAs and the RNAi Exp.html" target=_blank Jack Dixon protocol ( RNAi _Dixon.html" target=_blankdownloaded ...

Chemicals needed:Glucose L-( )-Arabinose Sigma Cat # A3256 L-Rhamnose Sigma cat # R3875 Cb (carbenicillin) Sigma cat # 1389DL-p-Chlorophenylalanine Sigma cat # C6506Media Recipes:YEG recombination pl ...