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Advances in understanding cancer genomes through second-generation sequencingCancers are caused by the accumulation of genomic alterations. Therefore, analyses of cancer genome sequences and structures provide insights for understanding cancer biology, diagnosis a ...

本人的想法：拿出自己研究过的文献（外文），供大家提出问题，讨论、翻译。相信大家的讨论一定会碰撞出新的ideas来。（已经搜索了相关内容，应该不是重复法帖，请班主支持！）相互交流、享受提高！！！Small Interfering RNA-Mediated Gene Silencing in TLymphocytes1Michael T. McManus, Brian B. Haines, Christopher P. Dillon, Charles E. Whitehurst,Luk van Par ...

When I read some posts, I often read some very basic questions. I always thinking, these questions are commoly taught by attending master or Ph.D courses. Or is easily solved by their tutors. Why they still ask such questions? So I write some of my thinkings, it may be benificial to new beginers. Due to my limited ti ...

The Scientist-September 23,2003-Fingering single genes-Cathy HoldingThe ability to control in vivo gene expression may be a powerful tool in cancer therapy, gene therapy of inherited genetic diseases, and for the study of gene function. Small interfering RNAs are increasingly used in ...

缺9卷Methods In Molecular Biology Collection - Volumes 101-200101-Mycobacteria Protocols-Methods in Mol Biol Vol 101.pdf102-Bioluminescence Methods and Protocols-Methods in Mol Biol Vol 102.pdf103-Pichia Protocols-Methods in Mol Biol Vol 103.pdf104-Mycoplasma Pro ...

Restriction Endonucleases: An OverviewRestriction enzymes were discovered about 30 years ago during investigations into the phenomenon of host-specific restriction and modification of bacterial viruses. Bacteria initially resist infections by new viruses, and this "re ...

刚刚看到的，基本上各种常见的和不常见的大肠杆菌表型在下面都可以查到，内容不是我写的，想成为分子生物学高手的网友应该好好研究一下。其内容来自：http://openwetware.org/wiki/E._coli_genotypesF- = Does not carry the F plasmidF+ = Carries the F plasmid. The cell is able to mate with F- through conjugation.F' = Carries an F plasmid that has host chr ...

http://www.jsbi.org/journal.htmlAt this first year of the new millennium, GIW has changed its name to the ``International Conference on Genome Informatics,'' while keeping GIW as its acronym. In the ``post-genome'' era, after the completion of the draft sequence of the human genome, we are faced ...

北京时间2014年7月30日，汤森路透《SCI期刊分析报告》（Journal Citation Reports）新鲜出炉，该份报告涵盖82个国家237个大类10927本期刊。今年公布的是这些杂志2013年的影响因子，今年新增了379种期刊，同时也剔除了33种期刊，因为它们自引率过高。影响因子在一定程度上的确是一本杂志质量高低的标准之一，并且能够带来科学以外太多的东西：教职、基金申请、学术影响力等。尽管很多学者批评过杂志的 ...

Mol Imaging. 2005 Oct-Dec;4(4):463-72.Micro-PET/CT monitoring of herpes thymidine kinase suicide gene therapy in a prostate cancer xenograft: the advantage of a cell-specific transcriptional targeting approach.Johnson M, Sato M, Burton J, Gambhir SS, Carey M, Wu L.University of Cali ...

转自：http://www.genscript.com/conversion.html一，Spectrophotometric Conversions1 A260 unit of double-stranded DNA=50 µg/ml1 A260 unit of single-stranded DNA=33 µg/ml1 A260 unit of single-stranded RNA=40 µg/ml二，DNA Molar Conversions1 µg of 1,000 bp DNA =1.52pmol(3.03pmol ...

这个主意真不错，制备一个能表达siRNA的文库，无需知道靶mRNA 的序列就可以筛选出有效的siRNAs，可以按照不同的要求筛选出许多有用的序列，从而节约了大笔money.采用病毒为载体，可以象PHAGE表面展示技术一样易于筛选。Small interfering RNAs (siRNAs) potently silence expression oftarget genes. In principle siRNA libraries can be used to perfo ...

siRNA DatabaseSearchable database of Silencer™ Validated and Pre-designed siRNAs to 34,000 human, mouse, and rat targets. All siRNAs in the database have been designed for maximum potency and specificity using a well-tested, proprietary algorithm. And silencing results are guaran ...

本内容需要回复后才能下载！ 本书版权归作者所有，请于下载后24小时内删除MicroRNA-从基础科学到疾病生物学（高清中英文版）0作　　者/Author:Krishnarao Appasani 著 Not Specified 编出 版 社/Publisher:Cambridge University PressＩＳＢＮ:9780521118552出版时间/Publication Date:2009-08-20版　　次/Edition:1页　　数/Pages:580MicroR ...

http://cmbi.bjmu.edu.cn/news/report/2002/cell-signal.htm Cell－signalhttp://cmbi.bjmu.edu.cn/news/report/2002/chemokine.htm chemokinehttp://cmbi.bjmu.edu.cn/news/report/2002/biochem.htm chembiochemhttp://cmbi.bjmu.edu.cn/news/re ...

主要有扫描电镜、离子溅射仪、临界点干燥仪 HCP－2、热分析仪、奥林巴斯系统显微镜（BX50 F）、毛细管电泳仪、气相色谱仪－II 、原子吸收分光光度（GBC）、荧光磷光分光光度计（LS55 型）、紫外可见分光光度计（GBC）、紫外可见分光光度计（GBC）使用方法、富里叶变换红外光谱仪、高效液相色谱仪（Waters 510）、极谱仪、液滴体积张力计（TVT2）、电泳系统（BIO RAD 3000）、UVP 凝胶成像系统、BIO RAD 低压层系系统、Geneamp PCRS ...

2013年，研究者们发表多篇文章介绍CRISPR/Cas9系统，但是CRISPR/Cas9目前仍存在一个很严重且无法避免的问题，即潜在的脱靶效应不可消除。 CRISPR/Cas9是由DNA切割酶Cas9和一段与靶 DNA片段匹配的20个核苷酸长度的短 RNA片段构成。它的特点在于只需合成一个gRNA就能实现对基因的特异性修饰，Cas9蛋白不具特异性；编码gRNA的序列不超过100bp，因此构建较简单方便。但是CRISPR ...

李再新 张富春（新疆大学生命科学与技术学院分子生物学重点实验室 乌鲁木齐 830046）自Wolff等1990年发现DNA能够刺激机体产生免疫效应以来,在全世界范围内迅速掀起了一股DNA疫苗研究热。在近十年的时间里,科学工作者在鼠、鸡、猪、牛、猫、猴和黑猩猩等动物中进行了广泛深入的抗病毒、抗细菌、抗寄生虫、抗肿瘤及免疫不育的DNA疫苗研究。由于DNA疫苗能诱导机体产生体液免疫和细胞免疫,并兼有减毒活疫苗的有效性和亚单位疫苗 ...

lncRNA专题讨论帖开始了！大家一起来交流与沟通吧！导语lncRNA在抗病毒天然免疫反应中具有重要调控功能。这一发现有助于深入了解病毒与宿主的相互作用，为新型抗病毒药物的研制提供新思路和重要的理论依据。该项研究成果于11月12日在国际学术期刊《细胞》子刊《细胞—宿主与微生物》（Cell Host &Microbe）上发表。流感病毒感染宿主后，病毒RNA被宿主的病原识别受体所识别，激活抗病毒天然免疫应答。一系列天然免疫相 ...

走出基因论的误区本文刊于《生物科学进展》1997；（6）：16-21。周慕瀛山东肥城矿务局中心医院271608摘要基因只是蛋白质分子的规格信息，它远不是物质本身．性状却必然意味着性状物质以及它的四维分布。从规格到实际物质的分布欠缺着有时、空坐标的物质制造活动．可见，基因根本决定不了性状的全过程。基因论的失误起始于孟德尔，延续到摩尔根并直至如今．生命的最大特色是主动性．牢记这一事实既易于走出基因论的误区，也易于找到生 ...