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miRNA是一类小分子RNARecent discoveries of small regulatory RNA molecules have been keeping the research community abuzz. Thus far, two classes of tiny regulatory RNA molecules have been identified.siRNAsThe first class encompasses the small interfering RNAs (siRNAs), which have an ...

Cloningof miRNAs was performed as described (Elbashir et al., 2001; Llaveet al., 2002a). In brief, ;600 mg of RNA was resolved through two laneson a denaturing 15% polyacrylamide gel. Labeled DNA oligonucleotideswere used as size standards. A gel fragment spanning the size rangeof 15 to 26 nucleo ...

1.核酸序列数据库•BIOSINO 我国的核酸序列公共数据库•EMBL - EMBL Nucleotide sequence database (EBI) *•GenBank - GenBank Nucleotide Sequence database (NCBI) *•DDBJ - DNA Data Bank of Japan *•dbEST - Database of Expressed Sequence Tags (NCBI)•dbSTS - Database of Sequence Tagged Sites (NCBI) 　2.基因组及相关数据库 Ge ...

Ethanol Precipitation of DNAEthanol precipitation is frequently used for concentration of DNA solutions and for removal of protein, salt, and unincorporated nucleotides. The two most common protocols use either 0.3 M sodium acetate (0.1 volume of 3 M) or 2.5 M ammonium acetate (0.5 volume of 7.5 M), ...

刚才在网上看到的，不知道是不是有人发过了。网址：http://www.csc.fi/oppaat/siru/下载地址：http://www.csc.fi/oppaat/siru/siruwww.pdf分部分下载地址：Part Ihttp://www.csc.fi/oppaat/siru/sirupartI.pdfPartIIhttp://www.csc.fi/oppaat/siru/sirupartII.pdfPartIIIhttp://www ...

RNAi GlossaryDicer - Dicer is a member of the RNase III family of nucleases that specifically cleave double-stranded RNAs. Dicer processes long dsRNA into siRNA of 21-23 nt.Interferon - A small and highly potent molecule that functions in an autocrine and paracrine manner, and that induces cel ...

复旦大学生化与分子生物学实验室黄伟达所率研究小组在Biochemical Journal的九月刊上报道了如题论文。哪位大侠能下载到全文，请在DXY里共享一下，以便大家交流学习。文摘如下：http://www.biochemj.org/bj/390/bj3900675.htmBiochem. J. (2005) 390 (675–679) (Printed in Great Britain)High doses of siRNAs induce eri-1 and adar-1 gene e ...

Nature Biotechnology 5年关于RNAi专题 :http://www.dxy.cn/bbs/post/view?bid=64&id=3130708&sty=1&tpg=1&age=01 Synthetic shRNAs as potent RNAi triggers2 Synthetic dsRNA Dicer substrates enhance RNAi potency and efficacy.◎3 Knockdown by RNAi-proceed with caution4 Rati ...

本人正在从事TILLING的研究,希望大家参与讨论.以下是几篇关于该技术的文献.大家可以自己到网上找,都能找到.找不到可以MAIL我,我可以传给大家.因为这里附件不能装那么多文献,所以大家可以直接找我要.1.McCallum C.M., Comai L., Greene E.A., Henikoff S. 2000 Targeted screening for ind-uced mutations. Nature Biotechnol. 18:455-72.McCallum C.M., Comai ...

看到一本权威的E文教科书关于gene knockout的过程，插图如下http://www.ncbi.nlm.nih.gov/books/bookres.fcgi/mcb/ch8f34.gifFigure 8-34. General procedure for producing gene-targeted knockout mice. Embryonic stem (ES) cells heterozygous for a knockout mutation in a gene of interest (X) a ...

1 RNAi实验原理、方法和应用http://www.dxy.cn/bbs/post/view?bid=64&id=1365323&sty=1&tpg=1&age=02 RNAi示意图http://www.dxy.cn/bbs/post/view?bid=38&id=1181258&sty=3&keywords=RNAi3 挺不错的RNAi的作用机制 ( FLASH )http://www.dxy.cn/bbs/post/view?bid=64&id=527666&s ...

由那位老师作过醛固酮合酶的RT-PCR，我已经P了两个月了，还是为扩出来。我用Wistar 大鼠的心肌及肾上腺组织提取RNA并RT，后进行PCR，已经换了3个引物，其中由两对引物是文献（英文一篇）报道的，但还未P出来，PCR条件我都试了好多次了，同样的标本P其他的3对基因都很顺利，就这对出不来，郁闷死了！！如果出不来结果以前的试验就白做了。下面是我的一对引物及基因序列：请帮忙分析一下，尤其是作过醛固酮合酶CYP11b PC ...

Chelex-100法提取微量DNA1. Add 1/50 volume of Proteinase K (10 mg/mL) and incubate the sample at 65°C for 2 hrs to overnight.2. 200 uL 5% Chexlex-100,add 10 uL proteinase K (20 mg/mL),inculate 2 hrs in 37 °C.3. 50ul Chelex液及lfl蛋白酶K水，56％温水浴消化2h后，煮沸8min，迅速放人冰盒冷却4min，以5000r／min速度离心lmin。吸取上清液 ...

宏基因组学Metagenomics Methods and Protocols基因X （美）（英文版）Metagenomics has proven to be a powerful tool for exploring the ecology, metabolic profiling, and comparison of complex microbial communities as well as its important applications in the mining of metagenomes for genes encodi ...

TOP10 chemically competent cells( modified from http://openwetware.org/wiki/TOP10_chemically_competent_cells#CCMB80_buffer)I also use this protocol to make Ecoli compent cell. It is pretty good.You can start from the fresh clone on plate, but you need a big clone or several clone ...

这一方法简单, 省钱, 不用酚/氯仿, 可用于酶切鉴定和转染细胞测试表达情况(尤其是直接观察表达情况的荧光表达载体). 具体操作步骤如下:Miniprep Plasmid Extraction1. Grow bacteria O/N at 37°C with vigorous aeration in 3 ml LB or 2xYT with appropriate antibiotic.2. Harvest by micro-centrifugation at maximum rpm (16000g) for 1 min (keep the 1 ml left ...

:)Genes and DiseaseBethesda (MD)本书汇编了关于基因与疾病关系方面内容的文章。基因疾病是由他们造成的人体的异常组成。同时，这种疾病影响身体的各个系统，可出现在不只一个章节Genes and Disease is a collection of articles that discuss genes and the diseases that they cause. These genetic disorders are organized by the parts of the body that ...

Ambion and Applied Biosystems have joined forces to provide a complete convenient, solution for performing gene silencing experiments and validating the results by real-time RT-PCR. Ambion's Silencer™ Validated and Silencer Pre-designed siRNAs eliminate the guesswork--and t ...

http://www.med.unibs.it/~marchesi/subjects.html其中的内容包括：Chemistry Review MaterialIonic Equilibria ReviewThermodynamics ReviewBasic Chemistry of BiomoleculesProtein StructureHemoglobinEnzyme Kinetics-Part IEnzyme Kinetics-Part IIVitam ...

下面的内容是我在浏览一些网站的时候发现的，感觉说的很通俗易懂，把多态性Polymorphism这个概念的方方面面呈现在了我们眼前，如果你对Polymorphism比较感兴趣，或者对这个概念还有疑惑，建议看一下，尽管是英文，但写得很值得一看。以下内容为网上搜集整理。Polymorphisms Genetic polymorphisms are different forms of a DNA sequence. "Poly" means many, and "mor ...