Single Nucleotide Polymorphisms: Methods and Protocols 第二版已经可以下载到了。奉献给大家。Single Nucleotide Polymorphisms: Methods and ProtocolsAnton A. Komar "Single Nucleotide Polymorphisms: Methods and Protocols"Humana Press | English | 2009-09-15 | ISBN: 1603274103 | 464 pages | PDF | ...
本内容需要回复后才能下载! 本书版权归作者所有,请于下载后24小时内删除基因的分子生物学(2013高清英文版)Molecular Biology of the Gene, 7th Ed名称Molecular Biology of the Gene作者James D. Watson, Tania A. Baker, Stephen P. Bell, Alexander Gann,Michael Levine, Richard Losick版本7, 插图版出版商Benjamin-Cummings Publishing C ...
各位师兄师姐,本人在实验当中碰到一问题,不知道如何让解释好,想请教一下。实验设计向一肿瘤细胞株中转入一外源基因,采用瞬时转染的方法,流式检测对细胞周期的影响,结果如下:转染48hG0/G1(%)S(%)G2/M(%)未转染组62.1630.117.72空载体组52.3828.7818.84目的基因组54.1520.8525.00G0/G1(%)S(%)G2/M(%)未转染组65.8529.424.73空载体组53.4034.4012.21目 ...
Some DNA Replication animations:http://www2.kenyon.edu/Depts/Biology/courses/biol14/slides/slides-2.htmlhttp://www.irn.pdx.edu/~newmanl/DNArepl1.movhttp://www.mhhe.com/biosci/genbio/animation_quizzes/graphics/lem6s2b.ramFrom t ...
第一作者之一的宋尔卫(Erwei Song)是我们熟知的,他是中山大学附二院的医生,现在哈佛大学医学院血液研究中心Judy Lieberman教授组进行博士后研究。他曾发表用尾静脉快速大剂量法向小鼠体内输注siRNA成功实现耐受抗Fas单抗诱导的暴发性肝坏死。http://www.nature.com/nbt/journal/v23/n6/abs/nbt1101.html;jsessionid=EFF86FD58B3B573 ...
绝版分子生物学实用技术分享:I: 20 种用于各种DNA marker 制备的PLASMID:P1: 2k+1.5k+1k+800+700+600+500+400+300+200X2+100X4 (100BP ladder)P2: 3k+1.5k+1k+900+700+500+300X2 (200BP ladder 奇数)P3: 3k+2k+1.4k+1k+800+600+4X2 (200BP ladder 偶数) (P3\P4\P5 组合, 可拓展为1KB ladder)P4: 4k+2k+1.4k+1k+800+6 ...
Modified Yeast TransformationInoculate cells from an overnight culture into 50 ml YEPD and incubate at 30°C with shaking. Typically, add 0.1 to 0.2 ml saturated culture in the evening to get an of OD600 = 1 to 2 the next morning.Transfer enough of this culture to 300 ml YEPD to produce an OD6oo=0.2 (ca. 20 mls.). Inc ...
1.Human embryonic stem cells express a unique set of microRNAs • ARTICLEDevelopmental Biology, Volume 270, Issue 2, 15 June 2004, Pages 488-498Mi-Ra Suh, Yoontae Lee, Jung Yeon Kim, Soo-Kyoung Kim, Sung-Hwan Moon, Ji Yeon Lee, Kwang-Yul Cha, Hyung Min Chung, Hyun Soo Yoon, Shin Yong Moon et al.SummaryPl ...
The race to the US$1,000 genome heated up today as Life Technologies, based in Carlsbad, California, announced that it will debut a new sequencing machine this year that will eventually be capable of decoding entire human genomes in a day for less than $1,000. The machine, called the Ion Proton, will be the s ...
下载方法:登陆 www.56.com注册邮箱后登陆在【共享资源beta】里输入 gtett123 即可Table of Contents1. Ribozyme- and siRNA-Mediated mRNA Degradaion: A General Introduction2. Combination of Chemical and Enzymatic RNA Synthesis3. Determination of Kinetic Parameters for Hammerhead and Hairpin Ribo ...
calcium phosphate transfection protocl:1.Split 293FT cells about 30% confluence, overnight.2.Thaw undefinedHBS room temperature (not in 37 C).3.For 10cm dish:1.5ml tube, 4ug packaging plasmid A, 4ug packaging plasmid B, 4ug expression plasmid, 61ul of 2M CaCl2,Mix properly, and then add distil ...
It is quite common to send small samples of plasmid DNA or other relatively stable material to other researchers. The most convenient method of doing this is to include it in a plain envelope mailed to the recipient at the cost of a first-class stamp. We have observed that it is common practice to pipet a few micr ...
走过很多弯路,也做了许多无用功,现在终于有了一点经验,拿出来请大家指教电转化protocol:a.incubation in MRS (10mL)with 1% glucose for 6 hours till OD600=0.4-0.6b.Pretreated with 10ng/mL Ampicillin for 60 minc.Washing with washing buffer two times, and then washing with electroporation buffer one time, resusp ...
做了好事——下载了,要留名啊!http://g.zhubajie.com/urllink.php?id=2594627fbvwb0br3hfv59kfPrefaceGene therapy and vaccination with nucleic acids is one of the most striking innovations in medical and veterinary sciences. The treatment of a disease on the level of genes rather than on t ...
DNA序列测定技术序列测定的技术和策略Sanger双脱氧链终止法Maxam-Gilbert DNA化学降解法测序策略 目前应用的两种快速序列测定技术是Sanger等(1977)提出的酶法及Maxam和Gilbert(1977)提出的化学降解法。虽然其原理大相径庭,但这两种方法都是同样生成互相独立的若干组带放射性标记的寡核苷酸,每组寡核苷酸都有固定的起点,但却随机终止于特定的一种或者多种残基上。由于DNA上的每一个碱 ...
生物芯片发展至今有很多名称和类型,通常将样品的制备,生化反应、结果的检测和分析这三步不同步骤集成为不同用途的生物芯片,据此分为不同的类型。例如用于样品制备的生物芯片,生化反应生物芯片及各种检测用生物芯片等。基因芯片技术发展的最终目标是将从样品制备、杂交反应到信号检测的整个生化检测分析过程集成到芯片上以获得微型全分析系统(micro total analytical system)或称所谓的“芯片实验室”(Lab-on-Ch ...
丁香园医学网站创立近三年,其间积累了大量的文献资料和信息资源,但大部分都分散在各个专业版块,难以给人以整体化、综合化的认识。为了更方便大家总结、利用这些资料信息,我们很早就着手准备《丁香园医学电子期刊》(暂定名)的筹备工作。《丁香园医学电子期刊》(暂定名)从逃、hashimoto等主任的初建,到yangster主任从多个角度的完善,这其中得到了许多热情的战友、主任们的大力支持并提出许多有建设性的建议,特别是版主yan ...
20万元转让DNA抽提系列试剂盒本试剂盒是由复旦大学博士后研制。考虑到目前国内大多数实验室研究经费的实际情况,我们力求研制出性价比比较高的试剂盒。配方、生产工艺、生产材料经过一系列优化,操作方便、生产设备简单、生产材料价廉。生产成本是目前国内最低的。用该系列试剂盒分离、纯化的DNA纯度不亚于国外同类试剂盒,而且DNA的得率较高。经过三年的销售使用,客户反映该系列试剂盒质量可靠、价格低廉,是目前市场上性价比最高的D ...
北京天来生物医学科技有限公司极限低价,全面促销!!!价格一步到位, 何必再谈折扣!无与伦比的低价, 绝不亚于同类产品的质量!效果不理想,无条件退货!一.DNA分子量标准每支(50次)---------仅售 58元!!!二.PCR反应混合液(PCR MasterMix)----500ul 68元三.质粒小提每次1.58元 胶回收每次1.78元四.中范围蛋白质分子量标准(14.3-97.2KD)(50次)------每支78元预染的宽范围蛋白质分子量标准(19-119KD) (5 ...
寄主蛋白指导农杆菌介导植物转化农杆菌在植物转化中已被广泛应用。因为起自然的转移外源DNA到寄主基因组的能力。它同时也是一个重要的植物病原物,这个病原体在几个农杆菌重要的物种中导致crown gall disease ,包括葡萄树、石榴、坚果树。在侵染寄主植物后,细菌从他肿瘤诱导的质粒中转移几种VIR蛋白和T-DNA到寄主细胞。在VIR蛋白的协助下,TDNA转移到寄主细胞的核中并且整合进寄主基因组中,当致癌基因在TDNA ...