全部

http://www.protocol-online.org/一个非常棒的实验技术资料提供网址。版面清晰，一目了然，内容丰富，各方面的生物学实验技术均有权威的PROTOCOL提供。主要内容如下：Animal TechniquesPathology, SurgeryBiochemistryAmino Acids & Protein, Lipid...BioinformaticsDatabase Search, Sequence Analysis...Cell ...

Kosmotropes and ChaotropesThe terms 'kosmotrope' (order-maker) and 'chaotrope' (disorder-maker) originally denoted solutes that stabilized, or destabilized respectively, proteins and membranes. Later they referred to the apparently correlating property of increa ...

Protocols 1In-gel Digestion of Coomassie Stained Proteins(No Reduction/Alkylation)Always wear gloves and work in the hood to reduce keratin contamination.Excise gel bands or spots.It is highly recommended that a positive and negative control be conducted in parallel to all analy ...

刚读到一片essay。觉得很有意思。”没有什么比生物医学研究令人更激动人心，或者说对社会更有利了。但具有讽刺意味的是，从来没有比现在更难成为一位生物医学研究工作者。“Jonathan W. Yewdell （他培养了至少13个PI）用这个开始了他对年轻科学家的指导。Biomedical research has never been more intellectually exciting or practically important to society. Iron ...

为什么蛋白质是边缘稳定的？以下是一篇文章的Ａbstract:Most globular proteins are marginallystable regardless of size or activity. The mostcommon interpretation is that proteins must bemarginally stable in order to function, and so marginalstability represents the results of positivesel ...

Subcloning NotebookSubcloning is a basic molecular biology procedure used to move inserts from one vector to another. Essentially all subcloning reactions proceed as follows: You release and purify your insert from the parent vector, ligate the insert into a prepared destination vec ...

20种氨基酸的结构与功能特点Glycine1. Gly存在于Pr中一般都有作用，因其容易destabilize Pr2. Contact point of α helices (prior to N-terminus, 因为易形成turn)3. C-cap of α helices4. Flattening of antiparallel β sheets5. Interior of parallel barrels6. Nucleotide binding motifs (GxGxxG)Proline1. 虽然疏水，但是经 ...

核酸、核苷酸的基本换算1．核酸的换算：(1)摩尔数与质量：1 μg 1,000bp DNA = 1.52 pmol1 μg pUC18/19 DNA (2,688bp) = 0.57 pmol1 μg pBR322 DNA (4,361bp) = 0.35 pmol1 μg SV40 DNA (5,243bp) = 0.29 pmol1 μg FX174 DNA (5,386bp) = 0.28 pmol1 μg M13mp18/19 DNA (7.250bp) = 0.21 pmol1 μg l phage DNA (48,502bp) = 0.03 pmol1 pmol 1,000bp DNA = 0.66 μg1 pmol pUC18/19 DNA (2 ...

Peter Järver and Ülo Langel. The use of cell-penetrating peptides as a tool for gene regulation Drug Discovery Today (DDT). 2004, 9(9): 395-402.The use of cell-penetrating peptides as a tool for gene regulationA novel carrier system that originates from membrane shuttling proteins such as the D ...

http://www.bio.com/store/product.jhtml?id=prod2020003Protein ArraysMethods and ProtocolsEric Fung, ed.HardcoverPublished April 2004304 PagesISBN: 1-58829-255-XA diverse collection of unique methodologies to synthesize and construct protein arrays for b ...

369_Electron_Microscopy_Methods_and_Protocols.rar375_In_Vitro_Transcription_and_Translation_Protocols_Second_Edition.rar380_Immunological_Tolerance_Methods_and_Protocols.rar379_Glycovirology_Protocols.rar367_Mass_ ...

商品名称规格数量单价0.5ml离心管1000支/包1180.001.5 ml离心管500支/包1100.002.0 ml离心管500支/包1110.00非变性裂解液100ml1160.00ECL超敏发光液 25ml1160.00WB膜封闭液50ml140.00考马斯亮蓝G-25010g1130.00甘氨酸500g178.00丽春红s10g180.00预染低范围蛋白质分子量标准（30-80KD）20次1320.00Bradford ...

Western blotting is a commonly used technique to study protein structure and function. Typically, protein samples are electrophoresed on SDS-gels and transferred to solid support (nitrocellulose or PVDF membranes) for subsequent probing with specific antibodiesRecycling of ...

要做肌浆网上的一蛋白，用RIPA试剂来从组织里提取；按它的说明如下：1.每100mg组织加入1mlRIPA和10ulPMSF（不能预先加到RIPA种，否则PMSF失效）。样本以玻璃匀浆器匀浆，埋在冰里，间或匀浆，避免起泡沫和发热，冰里匀浆；2.将样本转移到1.5ml离心管，4度高速离心30分钟。高速20000g低温离心。3.将上清小心转移到干净无菌离心管中，－20度保存。4.抽提的样本蛋白浓度可以用Lowry 法。空白对照采 ...

有7M，不能直接作为附件提供给大家，只能上传到FTP了。Chapter 1The Regulation of Gene Expression . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1Chapter 2The Regulation of Enzyme Activity . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 89Chapter 3Function and Structure of Signaling Pathways. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 119Chapter 4Signaling by Nuclear Receptors . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .

Pevzner教授 Do-It-Yourself 蛋白质组学课程Budding bioinformaticists in Pavel Pevzner's undergraduate research experiences class looked at the proteins found in a bacterium and worked backward to identify the genes that created them.The course was not for the faint of heart. Undergradua ...

http://rapidshare.com/files/11617014/catccama.rar.htmlHardcover: 507 pagesPublisher: John Wiley & Sons (August 30, 2006)Language: EnglishISBN: 3527312803Table of Contents1 Molecules with holes - cyclodextrins 12 Modification reactions of cyclodextrins and t ...

关于内参，已经有很多的帖子讨论，但是在我们的动物或细胞的模型中，总是有各种干扰的过程常常影响内参的表达，比如GAPDH就参与糖代谢，变性性疾病，凋亡等多种过程，如果我们的试验中存在这种过程干扰内参的表达，应该如何选择呢？Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) antibodyGlyseraldehyde-3-phosphate dehydrogenase (GAPDH) is a ca ...

　基因组(genome)包含的遗传信息经转录产生mRNA，一个细胞在特定生理或病理状态下表达的所有种类的mRNA称为转录子组(transcriptome)。很显然，不同细胞在不同生理或病理状态下转录子组包含的mRNA的种类不尽相同。mRNA经翻译产生蛋白质，一个细胞在特定生理或病理状态下表达的所有种类的蛋白质称为蛋白质组(proteome)。同理，不同细胞在不同生理或病理状态下所表达的蛋白质的种类也不尽相同。蛋白 ...

（转贴）抗菌肽的研究进展青霉素的发现使人们对由病原微生物感染而引发的各类疾病不再束手无策,并由此发展了大量的β-内酰胺类抗生素,对保护人类健康作出了巨大贡献。但随着上述“传统抗生素”的广泛使用,不断产生出诸多新问题。如β-内酰胺类抗生素的过敏反应以及长期使用导致抗药菌株的产生。于是人们开始寻找新一代抗菌剂。近期的研究发现,某些阳离子型多肽具有广谱的抗菌活性,同时具有“传统抗生素”无法比拟的优越性:不会诱导抗药菌株 ...