Pathogen-Free Mouse Rederivation by IVF, Natural Mating and Hysterectomy
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The increased popularity of genetically modified animals in collaborative studies has stimulated the widespread interchange of mice among institutions with inconsistent health standards. While the presence of certain organisms may be tolerated at one institution, the impact on the studies at another may demand clean animals. The importance of a well-established veterinary health surveillance program and a list of organisms to be excluded from the facility, i.e., a Specific Pathogen Free (SPF) list, become critical for animal facility management and maintenance of institutional standards.
Most institutions allow direct entry of mice into a holding room only when animals come from production areas of recognized vendors with a known high health status maintained for many years. A different plan must be in place when mice are introduced from areas of a lower or unknown health status. Rederivation is the method by which laboratory animals can be “cleaned” or decontaminated of certain pathogens, including transmissible zoonotic diseases, before being introduced into barrier housing facilities.
