• 我要登录|
  • 免费注册
    |
  • 我的丁香通
    • 企业机构:
    • 成为企业机构
    • 个人用户:
    • 个人中心
  • 移动端
    移动端
丁香通 logo丁香实验_LOGO
搜实验

    大家都在搜

      大家都在搜

        0 人通过求购买到了急需的产品
        免费发布求购
        发布求购
        点赞
        收藏
        wx-share
        分享

        The Technique of In Situ Hybridization

        互联网

        369
        In situ hybridization (ISH) (also called “hybridization histochemistry” or “hybridization cytology”) was first described in 1969 by Gall and Pardue who used the technique to localize ribosomal DNA in Xenopus oocytes (1 ). In contrast to other techniques of hybridization (i.e., Northern or Southern blotting), ISH allows detection of specific DNA or RNA molecules in single cells, histological sections, or chromosomes. During the past decades, ISH has become a powerful tool in molecular biology and pathology, and has been widely applied to the localization of viral DNA, detection of expression of messenger RNA, and analysis of genes in chromosomes. This chapter describes the technique of ISH in some detail and also explains the pitfalls associated with its use.
        ad image
        提问
        扫一扫
        丁香实验小程序二维码
        实验小助手
        丁香实验公众号二维码
        扫码领资料
        反馈
        TOP
        打开小程序