• 我要登录|
  • 免费注册
    |
  • 我的丁香通
    • 企业机构:
    • 成为企业机构
    • 个人用户:
    • 个人中心
  • 移动端
    移动端
丁香通 logo丁香实验_LOGO
搜实验

    大家都在搜

      大家都在搜

        0 人通过求购买到了急需的产品
        免费发布求购
        发布求购
        点赞
        收藏
        wx-share
        分享

        Fluorescence In Situ Hybridization (FISH) Technique for the Micronucleus Test

        互联网

        360
        In recent years, cytogenetics in combination with molecular methods has made rapid progress, resulting in new molecular cytogenetic methodologies such as fluorescence in situ hybridization (FISH). FISH is a molecular cytogenetic technique used for the detection of specific chromosomal rearrangements and applicable to many different specimen types. It uses fluorescently labeled DNA probes complementary to regions of individual chromosomes. These labeled DNA segments hybridize with the cytological targets in the sample and can be visualized by fluorescence microscopy in interphase nuclei or on metaphase chromosomes. Here, we describe the FISH methodology with centromeric probes for human cells, which is used in combination with the cytokinesis-block micronucleus assay and which allows discrimination between mutagens inducing DNA breakage (clastogens) or chromosome loss (aneugens).
        ad image
        提问
        扫一扫
        丁香实验小程序二维码
        实验小助手
        丁香实验公众号二维码
        扫码领资料
        反馈
        TOP
        打开小程序