• 我要登录|
  • 免费注册
    |
  • 我的丁香通
    • 企业机构:
    • 成为企业机构
    • 个人用户:
    • 个人中心
  • 移动端
    移动端
丁香通 logo丁香实验_LOGO
搜实验

    大家都在搜

      大家都在搜

        0 人通过求购买到了急需的产品
        免费发布求购
        发布求购
        点赞
        收藏
        wx-share
        分享

        Spin Column chromatography

        互联网

        1111

         

        <center> <h2> Spin Column chromatography</h2> <p>  </p> <h3> <i>Used to removed unincorporated nucleotides from labelling reactions.</i></h3> <p>  </p> </center>

         



        - Prepare Sephadex G-50 (medium) by adding appropriate amount of dry beads to 100 ml TE buffer such that the beads will swell to 50 ml. Autoclave.
        1. Rock bottle gently to make a uniform slurry.

        2. Place a disposable 3ml chromatography column into a 15 ml round bottom tube. Add 2 ml of the slurry to the column.

        3. Spin the tube in swing out cups of the Jouan centrifuge, using the pre-programmed setting (to determine the right time and speed on a different machine, use isotope to see how long and how fast to spin without letting the free dCTP go through the column to a significant degree. Co-load some DNA to ensure that this indeed flows through under new conditions. We go about 1500rpm for about 90 seconds)

         

        4. Dump out flow-through. Put a 1.5ml microfuge tube with a cut-off cap into the bottom of the tube.

         

        5. Replace the column and add your 200 ul sample to the center of the top of the bead bed.

         

        6. Spin on the same program.

         

        ad image
        提问
        扫一扫
        丁香实验小程序二维码
        实验小助手
        丁香实验公众号二维码
        扫码领资料
        反馈
        TOP
        打开小程序