Detection of Penicillin Resistance in Streptococcus pneumoniae by a Seminested PCR Strategy
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The first appearance of clinically significant penicillin-resistant Streptococcus pneumoniae (pneumococcus) occurred in 1967 in Australia (1 ) and penicillin resistant and multiresistant pneumococci have subsequently spread globally and reached high prevalence in many countries (2 ).
