Expression of Proteins in E. coli Utilizing a Dual Promoter-Based Vector: pLACT7
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Most commonly used prokaryotic expression vectors fall into one of the following three categories:
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They have a low copy number and a low yield of single-stranded DNA,
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| 2. |
They have a high copy number and the ability to produce a high yield of single-stranded DNA, but require a suitable host to achieve an inducible protein expression; or
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| 3. |
They lack the ability to produce single-stranded DNA.
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