• 我要登录|
  • 免费注册
    |
  • 我的丁香通
    • 企业机构:
    • 成为企业机构
    • 个人用户:
    • 个人中心
  • 移动端
    移动端
丁香通 logo丁香实验_LOGO
搜实验

    大家都在搜

      大家都在搜

        0 人通过求购买到了急需的产品
        免费发布求购
        发布求购
        点赞
        收藏
        wx-share
        分享

        Direct Cloning of Full-Length Cell Differentially Expressed Genes by Multiple Rounds of Subtractive Hybridization Based on Long-Distance PCR and Magne

        互联网

        449
        Subtractive cloning is an ideal technique for identifying genes differentially expressed in two nuclear acids population (1 ). The polymerase chain reaction (PCR)-based subtraction is the method of choice when the starting samples are heterogeneous or difficult to obtain, which often occurs in the tissues to be compared. PCR amplification is the easiest method for generating adequate amount of nuclear acids for multiple-round hybridization. However, the bias in the relative representation of mRNA molecules in the starting materials and the accumulation of shorter fragments become the major deficiencies for this method and should be overcome. The bias caused by PCR amplification is because of the tendency of preferentially amplifying short fragments and certain templates with unique sequences in the sample. The thermophilic polymerase that is optimized to amplify multiple genes would be helpful and the adoption of gel filtration in preparation of templates for amplification could hinder the tendency of short fragment accumulation. In addition, increasing the amount of starting samples would represent much more molecules in tissues.
        ad image
        提问
        扫一扫
        丁香实验小程序二维码
        实验小助手
        丁香实验公众号二维码
        扫码领资料
        反馈
        TOP
        打开小程序