Applications of Polysaccharide-Based Chiral Stationary Phases for Resolution of Different Compound Classes

The impact of chirality on drug development and use has been well documented (1 –6 ). Therefore, the chiral resolution is essential in pharmaceutical, agriculture, and food industries. In view of this, the U.S. Food and Drug Administration has issued certain guidelines for the marketing of racemic compounds (7 ). In last two decades, high-performance liquid chromatography (HPLC) has become one of the most applied modalities in the chiral resolution of different racemates (8 ,9 ). The most important aspect in chiral resolution by HPLC is the development of several chiral stationary phases (CSPs). The important CSPs include native or derivatized amino acids, derivatized polysaccharides (cellulose or amylose), cyclodextrin and its derivatives, protein phases, chiral crown ethers, macrocyclic antibiotics, and other chiral compounds (10 ). Among these, polysaccharide-based CSPs are very important, as they have achieved a great reputation in the field of chiral resolution. The importance of polysaccharide-based CSPs include their ease of use, reproducible results, and wide range of applications (8 ,9 ,11 –13 ).