• 我要登录|
  • 免费注册
    |
  • 我的丁香通
    • 企业机构:
    • 成为企业机构
    • 个人用户:
    • 个人中心
  • 移动端
    移动端
丁香通 logo丁香实验_LOGO
搜实验

    大家都在搜

      大家都在搜

        0 人通过求购买到了急需的产品
        免费发布求购
        发布求购
        点赞
        收藏
        wx-share
        分享

        Fluorescent Amplified Fragment Length Polymorphism Genotyping of Bacterial Species

        互联网

        520
        High-resolution and reproducible whole genome methodologies are needed as tools for rapid and cost-effective analysis of genetic diversity within bacterial genomes. These should be useful for a broad range of applications such as identification and subtyping of microorganisms from clinical samples, for identification of outbreak genotypes, for studies of micro- and macrovariation, and for population genetics.
        Fluorescent amplified fragment length polymorphism analysis is one such technique that has been used successfully for studying several bacterial genera. It combines the principle of restriction fragment length polymorphism with the capacity to sample bacterial genomes by selective amplification of a subset of DNA fragments generated by restriction endonucleases, thereby sampling multiple loci distributed throughout the genome. Typically, the genomic DNA is digested with two restriction endonucleases, followed by ligation of double-stranded oligonucleotide adaptors to ends of restriction fragments. Subsets of these fragments are amplified by PCR using fluorescently labeled primers complementary to the adaptor sequences. Amplified fragments are resolved by electrophoresis on an automated DNA sequencer and precisely sized using internal size standards in each sample.
        ad image
        提问
        扫一扫
        丁香实验小程序二维码
        实验小助手
        丁香实验公众号二维码
        扫码领资料
        反馈
        TOP
        打开小程序