Reprogramming to Pluripotency and Differentiation of Cells with Synthetic mRNA
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Changing cell fate without altering its genome is very desirable in many experimental systems and for cell therapy. Compared to DNA plasmid or viral-based approach, mRNA has the advantage of high transfection efficiency, no danger of changing the genome or creating mutational insertions. Here, we describe a straightforward protocol to synthesize mRNA of genes of interest, and use them to induce pluripotency and direct cell differentiation.
