Ca2+ Microfluorimetry in Retinal Mller Glial Cells

Calcium acts as a prominent second messenger in virtually every cell type and modulates a plethora of cell functions. Thus, Ca²⁺ microfluorimetry became a valuable tool to assess information about mechanisms involved in the regulation of the intracellular calcium level in research on living tissues. Here we offer insight into distinct approaches to detect changes in calcium levels specifically in M�ller cells, the principal macroglial cells of the retina.