Isolation of Total and Bioactive Interleukins by Immunoaffinity-Receptor Affinity Chromatography

The isolation of total and bioactive cytokines can be achieved by a combination of immunoaffinity and immobilized receptor chromatography. The former procedure isolates the total amount of a specific cytokine, and the latter procedure isolates the bioactive fraction of the immunoaffinity-purified material. Recombinant receptors are becoming available for a number of different biologic materials and are available in forms suitable for affinity chromatography.