Amplification of Lectin-Gold Histochemistry

Colloidal gold was developed as a probe for microscopy in the late 1970s. However, Roberts (1 ) had previously injected gold salts in living animals and detected the tissue-gold interactions microscopically by a photochemical method. With this method, metallic silver was deposited around gold particles Roberts based his method on the idea of Liesegang and Rieder (2 ), who visualized silver compounds injected into animals by a photographic process.