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        组织学一般技术

        互联网

        1694

         

         

        The manuals for this section have been compiled by Susan L. Wall, HT(ASCP). These manuals are in Adobe Acrobat PDF format and require the Acrobat Reader for viewing.

        Staining Manuals

        Fixative Solutions: Each of the following .PDF files contains the procedure plus a sample container label.

        95% Alcoholic Bouin's Fixative

        95% Alcohol Fixative

        B5 Fixative

        Bouin's Fixative

        Formalin Fixative

        Karnovsky's Fixative (glutaraldehyde)

        Hartman's Fixative

        Hollande's Fixative

        Orth's Solution (dichromate fixative)

        Zenker's Fixative

        Stock Solutions: Each of the following .PDF files contains the information regarding the use and preparation of a working solution.

        Acid cleaning solution for glassware

        Bluing solution for hematoxylin stain

        Celloidin slide adhesive

        Chrome alum adhesive

        Glycerin water mounting medium

        H and E acid decolorizing rinse

        Hypo (sodium thiosulfate)

        Lugol's iodine for mercury removal

        Metal embedding mold release

        Paraffin block softening solution

        Miscellaneous Labels

        Staining Procedures: Each of the following .PDF files contains the procedure for a special stain, a procedure card, and sample container labels for the reagents.

        Introduction

        Acid Fast Stain (for mycobacteria)

        Acid Fast Stain (Ziehl-Neelsen, for M. leprae and Nocardia) [contributed by Greg Winn, Specialist Histopathology & Cytology Services, Western Australia]

        Fite's Acid Fast Stain

        Alcian Blue Stain

        Alcian Blue-PAS Stain (PAB)

        Hyaluronidase Digestion for Alcian Blue

        Alizarin Stain for calcium

        Auramine-Rhodamine Stain (fluorescent)

        Bielschowsky Stain (for senile plaques)

        Bile Stain

        Bodian's Stain

        Colloidal Iron Stain

        Congo Red Stain

        Copper Stain

        Elastic van Gieson Stain

        Elastic - Weigert's resorcin-fuchsin method

        Modified Elastic van Gieson Stain

        Fontana-Masson Stain for melanin

        Melanin Bleach

        Fraser Lendrum Stain

        Giemsa (Modified May-Gruenwald) Stain (for hematopoietic tissues)

        Giemsa Stain (for Helicobacter)

        Gram (Modified Brown-Brenn) Stain

        Gridley's Stain for ameba

        Grimelius Argyrophil Stain (Pascual's Method)

        Grocott's Methenamine Silver (GMS) Stain

        Holzer's Glial Fiber Stain

        Hortega's Pineal Stain

        Iron Stain (Prussian blue)

        Iron Stain (Turnbull's blue)

        Jones' Silver Stain

        Luxol Fast Blue (LFB) Stain

        Methyl Green Pyronin (MGP) Stain

        Mucicarmine Stain

        Nissl Stain

        Oil Red O Stain

        Orcein Stain

        Periodic acid-Schiff Stain (PAS)

        Periodic acid-Schiff, digested Stain (PAS-D)

        PTAH Stain

        Reticulin Stain

        Spirochete Stain (Steiner & Steiner method)

        Sudan Black B Stain (for lipochrome)

        Sudan Black B Stain (for fat)

        Trichrome Stain - Masson's method

        Trichrome Stain - microwave method

        Thioflavin S Stain (for amyloid in tissues)

        Modified Thioflavin S Stain (for senile plaques)

        Toluidine Blue Stain (for mast cells)

        Urate Crystal Stain

        VonKossa Stain for calcium

        Safety Procedures

        Bloodborne Pathogens (Exposure Control Plan)

        Working with Chemicals

        Chemical Hygiene Safety

        Chemical Inventory

        Emergency Procedures

        Facilities (engineering controls)

        Safety Equipment (engineering controls)

        Training Records

        Tuberculosis Guidelines

         

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