DNA Microarrays for Gene Expression Analysis in Brain Tissue and Cell Lines
互联网
441
Microarray expression profiling of the nervous system provides a powerful approach to ascribe activities to genes involved
in distinct phases of neural development and function. Expression profiling of neural tissues and cell lines requires isolation
of high-quality RNA, amplification of the isolated RNA, and hybridization to DNA microarrays. In this chapter, theoretical
background for expression profiling as well as protocols for reproducible microarray experiments from brain tissue and cell
lines derived from brain tissue will be presented in the context of neural proliferation and tumorigenesis in the cerebellum.