Fluorescent Glycerolipid Probes: Synthesis and Use for Examining Intracellular Lipid Trafficking
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The synthesis, characterization, and use of fluorescently labeled lipid probes to follow transport in living cells at a microscopic level have greatly expanded our knowledge of intracellular lipid trafficking. Although lipids containing a number of covalently attached fluorophores have been synthesized, most transport studies have used lipids labeled with 4-nitrobenzo-2-oxa-l,3-diazole (NBD).** Several lines of evidence suggest that NBD-labeled lipids faithfully mimic their native counterparts (see ref. 1 for review). For example:
