• 我要登录|
  • 免费注册
    |
  • 我的丁香通
    • 企业机构:
    • 成为企业机构
    • 个人用户:
    • 个人中心
  • 移动端
    移动端
丁香通 logo丁香实验_LOGO
搜实验

    大家都在搜

      大家都在搜

        0 人通过求购买到了急需的产品
        免费发布求购
        发布求购
        点赞
        收藏
        wx-share
        分享

        PACE Analysis of RNA-Peptide Interactions

        互联网

        510
        The PACE assay is a relatively new addition to the arsenal of techniques used to examine quantitatively the interactions of proteins and peptides with DNA and RNA (1 ). Polyacrylamide coelectrophoresis (PACE) involves electrophoresis of a labeled nucleic acid through a gel medium that contains the target peptide or protein ligand. In this way, the nucleic acid is maintained in a constant concentration of the ligand throughout the electrophoresis, and the conditions for binding equilibrium are maintained throughout the experiment. This avoids the requirement for formation of complexes that are kinetically stable under the nonequilibrium conditions typical of a gel mobility shift experiment. A particularly powerful aspect of the PACE experiment is the ability to probe interactions that are too weak to be observed in other binding assays such as gel shift or filter binding (see Chapters 9 and 10 ).
        ad image
        提问
        扫一扫
        丁香实验小程序二维码
        实验小助手
        丁香实验公众号二维码
        扫码领资料
        反馈
        TOP
        打开小程序