The Gibson Assembly Song
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Gibson Assembly is a high-efficiency DNA end-linking technique developed by Daniel Gibson at the JCVI in 2009 [Gibson et al. 2009 plus supplementary methods]. The technique was invented and perfected as part of the genome assembly efforts at JCVI. The method uses three enzymes to join two or more sequences of DNA when they have overlapping end sequences at their joining point (~40bp). These overlapping regions can be easily added to the ends of any length of DNA by using PCR with primers which have added adapter sequences. Thus PCR followed by Gibson Assembly allows you to join any two blunt ended pieces of DNA. Up to 10-20 different pieces of DNA can be neatly spliced together in one reaction using these techniques.
