Analysis of Genetic Variants in Cardiovascular Risk Genes by Heteroduplex Analysis
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As an increasing number of human diseases are linked to the effects of altered genes, new methods are being sought for detection of mutations and their relationship to the presence of disease. Since total genomic DNA usually cannot be analyzed directly, target sequences are amplified by the polymerase chain reaction (PCR). Several methods have been reported that allow detection of small changes in DNA sequence (1 ). Among them, one of the most commonly used is the heteroduplex analysis method (HA) (2 –5 ).
