Computer Analysis of 2-D Images
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Two-dimensional polyacrylamide gel electrophoresis (2-D PAGE) images, when used to analyze protein expression in large sets of biological samples, are best processed by computer. In this manner, one can detect and quantify even the faintest spots, quantitatively compare 2-D images among each other, or identify protein expression changes across various sets of 2-D images, such as disease vs control, or different stages in a toxicological study (1 ).
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