Gold NanoparticleOligonucleotide Conjugates for the Profiling of Malignant Melanoma Phenotypes

This chapter discusses the preparation and subsequent profiling capabilities of gold nanoparticle-oligonucleotide conjugates for multiple melanoma mRNA targets. We will outline the attachment of DNA hairpins modified with a thiol for facile attachment to gold nanoparticle surfaces through gold-sulfur bond formation. Furthermore, the ability of these conjugates to detect and distinguish phenotypic variations utilizing �several melanoma cell lines and the nonmalignant cell line, HEp-2, will be investigated using flow cytometry and RT-PCR analytical techniques. The behavior of the housekeeping probe β-actin will also be investigated as a control.