• 我要登录|
  • 免费注册
    |
  • 我的丁香通
    • 企业机构:
    • 成为企业机构
    • 个人用户:
    • 个人中心
  • 移动端
    移动端
丁香通 logo丁香实验_LOGO
搜实验

    大家都在搜

      大家都在搜

        0 人通过求购买到了急需的产品
        免费发布求购
        发布求购
        点赞
        收藏
        wx-share
        分享

        无血清培养基培养乳猪肝细胞的效果

        互联网

        750
        相关专题
         

        陈钟,丁义涛,南京大学 医学院附属鼓楼医院肝胆外科 江苏省南京市 210008
        张鹤云,南京大学生化系 江苏省南京市 210093
        陈钟,男,1963-07-04生,江苏省靖江市人,汉族.1983年南通医学院医疗系本科毕业,1988年南通医学院外科硕士研究生毕业,1999年南京大学医学院肝胆外科博士生,副教授,副主任医师,主要从事肝胆外科及生物人工肝研究.
        江苏省 卫生厅重点发展项目基金资助课题 No. BQ200020
        项目负责人
        陈钟,210008,江苏省南京市中山路321号,南京大学医学院附属鼓楼医院肝胆外科. zchen9999@sina.com
        电话: 025-3304616 传真:025-6635721
        收稿日期 2001-12-04 接受日期 2002-01-04

         


        Serum-free medium for culture of suckling pig hepatocytes

         

        Zhong Chen, Yi-Tao Ding, He-Yun Zhang

         

        Zhong Chen, Yi-Tao Ding, Department of Hepatobiliary Surgery, Affiliated Drum Tower Hospital, Medical School, Nanjing University, Nanjing 210008, Jiangsu Province, China
        He-Yun Zhang,Department of Biochemistry, Nanjing University, Nanjing 210093, Jiangsu Province, China
        Supported by the Key Development Foundation of Health Department of Jiangsu Province, No. BQ200020
        Correspondence to:
        Zhong Chen, Department of Hepatobiliary Surgery, Affiliated Drum Tower Hospital, Medical School, Nanjing University, Nanjing 210008, Jiangsu Province, China. zchen9999@sina.com
        Received 2001-12-04 Accepted 2002-01-04

         


        Abstract

         

        AIM: To compare the effect of serum-free medium with serum-contained medium for culture of sucking pig hepatocytes and to search for a kind of better serum-free medium for bioartificial liver.

         

        METHODS: Suckling pig hepatocytes were isolated by modified two-step in situ collagenase perfusion method and cultured at 5×108·L-1 both in serum-free medium and serum-contained medium, respectively. Viability, protein and glucose syntheses, G-6-Pase activity, diazepam transformation of hepatocytes and LDH release were evaluated during 7d of culture.

         

        RESULTS: There were no significant differences in above indices except for lower G-6-Pase activity on 0, 1, 2d and glucose synthesis on 2,7d. High viability (>88%) was obtained though it declined with time. Protein synthesis of hepatocytes cultured in serum-free medium was stable over the course of 7d. Diazepam transformation of hepatocytes was stronger on 2d and 3d (diazepam concentration was 75±4pg·Cell -1 on 2d and 77±5pg·cell-1 on 3d). Glucose synthesis of hepatocytes declined from 1.00±0.15nmol·cell-1 on 1d to 0.71±0.02nmol·cell-1 on 2d. G-6-Pase activity of the hepatocytes declined apparently from 1290±30zmol·cell-1 on 0d to 306±38zmol·cell-1 on 1d, and it was maintained at low level for 7d. LDH release was higher on 2d and 3d.

         

        CONCLUSION: The serum-free medium may be applied in the culture of porcine hepatocytes of bioartificial liver.

         

        Chen Z, Ding YT, Zhang HY. Serum-free medium for culture of suckling pig hepatocytes.
        Shijie Huaren Xiaohua Zazhi 2002;10(3):320-323

         

        ad image
        提问
        扫一扫
        丁香实验小程序二维码
        实验小助手
        丁香实验公众号二维码
        扫码领资料
        反馈
        TOP
        打开小程序