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成纤维细胞培养基

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  • ¥2224
  • ScienCell已认证
  • 2301
  • 美国
  • 2026年04月27日
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  • 企业认证

    • 详细信息
    • 询价记录
    • 文献和实验
    • 技术资料
    • 库存

      999

    • 供应商

      亿泽丰

    • 英文名

      Fibroblast Medium

    • 规格

      500mL

    成纤维细胞培养基是专门为正常人类成纤维细胞体外培养设计的最适于其生长的培养基。它是经灭菌的液体培养基,包含必需和非必需氨基酸、维生素、有机和无机化合物、激素、生长因子、微量矿物质和低浓度胎牛血清(2%)。该培养基缓冲体系为HEPES和重碳酸盐,在含5%CO2的细胞培养箱中平衡后pH值为7.4。该培养基的配方能够选择性的促进正常人类成纤维细胞体外培养中的增殖和生长,并为其达到最理想营养平衡状态提供数量上和质量上的保证。

     

        成纤维细胞培养基包含500mL基础培养基,10mL胎牛血清(FBS,NO.0010),5mL成纤维细胞生长添加物(FGS,NO.2352)和5mL青霉素/链霉素溶液(P/S,NO.0503)。

     

    产品使用说明:仅供科研研究使用!

     

    参考文献:

    1. Choi, Y., Hyun, E., Seo, J., Blundell, C., Kim, H.C., Lee, E., Lee, S., Moon, A., Moon, W.K. & Huh, D. (2015) A microengineered pathophysiological model of early-stage breast cancer Lab Chip. 15

    2. Inoue-Mochita, M., Inoue, T., Fujimoto, T., Kameda, T., Awai-Kasaoka, N., Ohtsu, N., Kimoto, K. & Tanihara, H. (2015) p38 MAP Kinase Inhibitor Suppresses Transforming Growth Factor-Beta2-Induced Type 1 Collagen Production in Trabecular Meshwork Cells PLoS One. 10

    3. Holton SE, Bergamaschi A, Katzenellenbogen BS, Bhargava R. (2014) 'Integration of Molecular Profiling and Chemical Imaging to Elucidate Fibroblast-Microenvironment Impact on Cancer Cell Phenotype and Endocrine Resistance in Breast Cancer.'

    4. Holton SE, Bergamaschi A, Katzenellenbogen BS, Bhargava R. (2014) "Integration of Molecular Profiling and Chemical Imaging to Elucidate Fibroblast-Microenvironment Impact on Cancer Cell Phenotype and Endocrine Resistance in Breast Cancer." PloS one 9: e96878.

    5. Li F, Weir MD, Chen J, Xu HHK. (2013) "Comparison of quaternary ammonium-containing with nano-silver-containing adhesive in antibacterial properties and cytotoxicity." Dent Mater. 29: 450-61.

    6. Williams M, Villarreal A, Bozhilov K, Lin S, Talbot P. (2013) "Metal and silicate particles including nanoparticles are present in electronic cigarette cartomizer fluid and aerosol." PLoS One. 8: e57987.

    7. Zhang K, Cheng L, Imazato S, Antonucci JM, Lin NJ, Lin-Gibson S, Bai Y, Xu HHK. (2013) "Effects of dual antibacterial agents MDPB and nano-silver in primer on microcosm biofilm, cytotoxicity and dentin bond properties." J Dent. 41: 464-74.

    8. Cavet ME, Volhejn S, Harrington KL, Zhang JZ. (2013) "Anti-allergic effects of mapracorat, a novel selective glucocorticoid receptor agonist, in human conjunctival fibroblasts and epithelial cells." Mol Vis. 19: 1515-25.

    9. Gong Y, Scott E, Lu R, Xu Y, Oh WK, Yu Q (2013) 'TIMP-1 Promotes Accumulation of Cancer Associated Fibroblasts and Cancer Progression.'

    10. Gong Y, Scott E, Lu R, Xu Y, Oh WK, Yu Q (2013) "TIMP-1 Promotes Accumulation of Cancer Associated Fibroblasts and Cancer Progression." PloS one. 8: e77366.

    11. Wang K, Zhang S, Weber J, Baxter D, Galas DJ. (2010) 'Export of microRNAs and microRNA-protective protein by mammalian cells.'

    12. Huntley BK, Ichiki T, Sangaralingham SJ, Chen HH, Burnett JC. (2010) 'B?type natriuretic peptide and extracellular matrix protein interactions in human cardiac fibroblasts.'

    13. Huntley BK, Ichiki T, Sangaralingham SJ, Chen HH, Burnett JC. (2010) "B?type natriuretic peptide and extracellular matrix protein interactions in human cardiac fibroblasts." J of cell physiol. 225: 251-55.

    14. Wang K, Zhang S, Weber J, Baxter D, Galas DJ. (2010) "Export of microRNAs and microRNA-protective protein by mammalian cells." Nucleic Acids Res. 38:7248-59.

    ScienCell Research Laboratories (SRL) takes pride in being a resource for researchers all over the world. The publications listed here are not meant as an endorsement or confirmation of the reliability of the research methods. Our sole intention of sharing the research publications listed here is to provide research related insights and innovations of our products with other researchers.

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    该产品被引用文献

    1. Choi, Y., Hyun, E., Seo, J., Blundell, C., Kim, H.C., Lee, E., Lee, S., Moon, A., Moon, W.K. & Huh, D. (2015) A microengineered pathophysiological model of early-stage breast cancer Lab Chip. 15

    2. Inoue-Mochita, M., Inoue, T., Fujimoto, T., Kameda, T., Awai-Kasaoka, N., Ohtsu, N., Kimoto, K. & Tanihara, H. (2015) p38 MAP Kinase Inhibitor Suppresses Transforming Growth Factor-Beta2-Induced Type 1 Collagen Production in Trabecular Meshwork Cells PLoS One. 10

    3. Holton SE, Bergamaschi A, Katzenellenbogen BS, Bhargava R. (2014) 'Integration of Molecular Profiling and Chemical Imaging to Elucidate Fibroblast-Microenvironment Impact on Cancer Cell Phenotype and Endocrine Resistance in Breast Cancer.'

    4. Holton SE, Bergamaschi A, Katzenellenbogen BS, Bhargava R. (2014) "Integration of Molecular Profiling and Chemical Imaging to Elucidate Fibroblast-Microenvironment Impact on Cancer Cell Phenotype and Endocrine Resistance in Breast Cancer." PloS one 9: e96878.

    5. Li F, Weir MD, Chen J, Xu HHK. (2013) "Comparison of quaternary ammonium-containing with nano-silver-containing adhesive in antibacterial properties and cytotoxicity." Dent Mater. 29: 450-61.

    6. Williams M, Villarreal A, Bozhilov K, Lin S, Talbot P. (2013) "Metal and silicate particles including nanoparticles are present in electronic cigarette cartomizer fluid and aerosol." PLoS One. 8: e57987.

    7. Zhang K, Cheng L, Imazato S, Antonucci JM, Lin NJ, Lin-Gibson S, Bai Y, Xu HHK. (2013) "Effects of dual antibacterial agents MDPB and nano-silver in primer on microcosm biofilm, cytotoxicity and dentin bond properties." J Dent. 41: 464-74.

    8. Cavet ME, Volhejn S, Harrington KL, Zhang JZ. (2013) "Anti-allergic effects of mapracorat, a novel selective glucocorticoid receptor agonist, in human conjunctival fibroblasts and epithelial cells." Mol Vis. 19: 1515-25.

    9. Gong Y, Scott E, Lu R, Xu Y, Oh WK, Yu Q (2013) 'TIMP-1 Promotes Accumulation of Cancer Associated Fibroblasts and Cancer Progression.'

    10. Gong Y, Scott E, Lu R, Xu Y, Oh WK, Yu Q (2013) "TIMP-1 Promotes Accumulation of Cancer Associated Fibroblasts and Cancer Progression." PloS one. 8: e77366.

    11. Wang K, Zhang S, Weber J, Baxter D, Galas DJ. (2010) 'Export of microRNAs and microRNA-protective protein by mammalian cells.'

    12. Huntley BK, Ichiki T, Sangaralingham SJ, Chen HH, Burnett JC. (2010) 'B?type natriuretic peptide and extracellular matrix protein interactions in human cardiac fibroblasts.'

    13. Huntley BK, Ichiki T, Sangaralingham SJ, Chen HH, Burnett JC. (2010) "B?type natriuretic peptide and extracellular matrix protein interactions in human cardiac fibroblasts." J of cell physiol. 225: 251-55.

    14. Wang K, Zhang S, Weber J, Baxter D, Galas DJ. (2010) "Export of microRNAs and microRNA-protective protein by mammalian cells." Nucleic Acids Res. 38:7248-59.

    ScienCell Research Laboratories (SRL) takes pride in being a resource for researchers all over the world. The publications listed here are not meant as an endorsement or confirmation of the reliability of the research methods. Our sole intention of sharing the research publications listed here is to provide research related insights and innovations of our products with other researchers.

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      !)。培养皿中可以先涂较薄的一层含血清培养基,再将组织块贴上去,密度高一些,便于细胞融合。4-10小时后加少量培养液,不要让组织块浮起!!以后几天不要动他,最快3-4天就可以有细胞爬出了! 细胞需要传代时,要先用无菌的小镊子将组织块夹出,再用0.25%胰酶(含0.02%EDTA)消化即可。 如果细胞来自瘢痕疙瘩,在DMEM培养2-3代之后就基本上全是成纤维细胞了,没有特殊原因不需要鉴定。 丁香园网友fanny816che的观点为: 材料:对遗传咨询门诊疑为Turner's征的患者取前臂内侧皮肤

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