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        Identification of Differentially Expressed Genes in Young and Senescent T Cells

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        The need to analyze changes in gene expression patterns occuring in senescent cells has stimulated the search for proper methods to identify the actual differences between young and senescent cells. In studies of aging, differential display reverse transcriptase-polymerase chain reaction (DDRT-PCR) has already been applied successfully by several groups. Altered gene expression in young and senescent fibroblast cultures (1 ), human mammary epithelial cells (2 ), and rat brain cells (3 , 4 ) has been detected with this technique.
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