MicroRNA Expression During Neuronal Differentiation of Human Teratocarcinoma NTera2D1 and Mouse Embryonic Carcinoma P19 Cells

MicroRNAs (miRNAs) are 21–23-nucleotide-long small noncoding RNAs, function as mediators in gene silencing, and play essential roles in gene regulation in development, differentiation, and proliferation. Hundreds of miRNAs have been found, and tissue-specific or organ-specific expression of miRNAs has been detected. Here, I describe procedures for detection of miRNAs in the course of neuronal differentiation of human teratocarcinoma NTera2D1 and mouse embryonic carcinoma P19 cells.