• 我要登录|
  • 免费注册
    |
  • 我的丁香通
    • 企业机构:
    • 成为企业机构
    • 个人用户:
    • 个人中心
  • 移动端
    移动端
丁香通 logo丁香实验_LOGO
搜实验

    大家都在搜

      大家都在搜

        0 人通过求购买到了急需的产品
        免费发布求购
        发布求购
        点赞
        收藏
        wx-share
        分享

        Random Primed Labeling

        互联网

        1035
        Labeling of DNA by nick translation has three major drawbacks: the time taken to perform the reaction (at least 1 h), the temperature sensitivity of the reaction, and the low specific activity of the probes generated. Random primed labeling developed by Feinberg and Vogelstein (1 ,2 ) solves all of these problems. The technique uses short random sequence hexanucleotides (in the original method) which prime the denatured target DNA at numerous sites. The Klenow fragment of DNA polymerase I is then used to synthesize new strands of DNA from these primed sites. The addition of a radioactive nucleotide results in a labeled probe suitable for use in Southern hybridizations, etc. Small amounts of starting material are required and the 10-min reaction results in probes labeled to a high specific activity.
        ad image
        提问
        扫一扫
        丁香实验小程序二维码
        实验小助手
        丁香实验公众号二维码
        扫码领资料
        反馈
        TOP
        打开小程序