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- 详细信息
- 文献和实验
- 技术资料
- 英文名:
Random Primed DNA Labeling Kit
- 供应商:
嵘崴达
- 保质期:
见产品外包装
- 保存条件:
−20°C
- 库存:
需确认
- 规格:
50 REACTIONS
随机引物 DNA 标记试剂盒
Random Primed DNA Labeling Kit
General description
随机引物 DNA 标记试剂盒是以随机寡核苷酸为引物,用修饰的脱氧核苷三磷酸对 DNA 进行放射性和非放射性标记。该方法通过 Klenow 聚合酶用 3′-作为引物的随机寡核苷酸的 OH 末端。
Specificity
热灭活:通过加入2μl 0.2MEDTA (pH 8.0) 和/或加热至 65°C 10 分钟来停止反应。
Application
Random Primed DNA Labeling Kit has been used to label probe or fragments shorter than 1 kb.
随机引物 DNA 标记试剂盒用于用任何 [α-32P] -dNTP 或修饰的 dNTP 均匀标记质粒或噬菌体 DNA。具有高比活性的标记 DNA 探针用于多种杂交技术,包括:
• 基因文库的筛选
• Southern、斑点 和 northern 印迹
• 原位 杂交
包装
1 个试剂盒包含 7 种组分。
| 11004760001 | Random Primed DNA Labeling Kit | 1 kit (50 labeling reactions) |
| 11207733910 | Anti-digoxigenin POD-conjugate | 150 U |
| 11442066001 | BM Blue POD Substrate, precipi | 100 ml |
| 11633716001 | Anti-Digoxigenin-POD (poly) | 50 U |
| 11585606910 | DIG High Prime 160ul | 160 µl (40 labeling reactions) |
| 11062590001 | DNA MW-Marker VI | 50 µg (1 A260 unit) |
| 11277073910 | DIG RNA Labeling Mixture, 10x | 40 µl (20 reactions) |
| 3353591910 | DIG Gel Shift Kit, 2nd generat | 1 kit |
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文献和实验Random primed labeling of DNA has now almost superseded the method of nick translation of DNA. Random primed labeling, based on the method of Feinberg and Vogelstein (1) is a method of incorporating radioactive nucleotides along the length
Labeling of DNA by nick translation has three major drawbacks: the time taken to perform the reaction (at least 1 h), the temperature sensitivity of the reaction, and the low specific activity of the probes generated. Random primed labeling
Random Primed 32P-Labeling of DNA
Random primed labeling of DNA has now almost superseded the method of nick translation of DNA. Random primed labeling, based on the method of Feinberg and Vogelstein (1 ), is a method of incorporating radioactive nucleotides along the length
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