• 我要登录|
  • 免费注册
    |
  • 我的丁香通
    • 企业机构:
    • 成为企业机构
    • 个人用户:
    • 个人中心
  • 移动端
    移动端
丁香通 logo丁香实验_LOGO
搜实验

    大家都在搜

      大家都在搜

        0 人通过求购买到了急需的产品
        免费发布求购
        发布求购
        点赞
        收藏
        wx-share
        分享

        Virus-Induced Gene Silencing in Soybean

        互联网

        540
        Understanding the biology of a system requires the association of gene functions to phenotypes and vice versa. Although a large number of resources and genomic tools are available for studies in the crop plant soybean, investigations related to gene function have been lacking. This is largely due to the fact that rapid functional genomics tools have been unavailable for application in soybean. This constraint was recently alleviated when a novel bean pod mottle virus (BPMV)-based system for virus-induced gene silencing in soybean was developed. This methodology exploits the plant’s ability to silence virus-encoded sequences as a defense mechanism. Plants infected with recombinant viruses carrying sequences that are identical/near-identical to the plant’s own genes suppress the expression of the target endogenous gene(s). Phenotypes associated with suppression of target sequence enable the assignment of functions to specific genes. The strategy involves inserting short sequences of target soybean genes into the viral genome to generate recombinant vectors. Soybean plants are then inoculated with the recombinant vectors, assessed for BPMV propagation and silencing of the target genes. Plants silenced for specific targets can then be analyzed for various different traits depending upon the known/predicted functions of the target genes. Using this strategy, we have identified genes participating in basal, resistance gene-mediated, and systemic immunity in soybean.
        ad image
        提问
        扫一扫
        丁香实验小程序二维码
        实验小助手
        丁香实验公众号二维码
        扫码领资料
        反馈
        TOP
        打开小程序