In Vitro Transcription of Class II Promoters in Higher Plants

Class II gene in vitro transcription systems from animal cells (1 –3 ) or fungi (4 ) have been essential for elucidating the mechanisms regulating gene expression. Crude protein extracts from Hela cell nuclei that allow accurate transcription of class II promoters in vitro have led to the investigation of DNA sequences required for initiation of transcription (cis -acting-factors) and protein factors that bind to these regulatory elements of promoters (trans -acting-factors).