Amplification Using Degenerate Primers with Multiple Inosines to Isolate Genes with Minimal Sequence Similarity
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The use of degenerate primers in the polymerase chain reaction (PCR) is an effective method for identifying related genes that share limited sequence similarity. Other methods, using oligonucleotide or heterologous probes, for example, may fail to identify genes that are not highly conserved, have diverged over evolutionary time, or share only short regions of structural similarity.
