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        Use of Self-Quenched, Fluorogenic LUX Primers for Gene Expression Profiling

        互联网

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        Application of a real-time detection system based on a novel primer design in gene expression profiling is described. In this system, called LUXTM (Light Upon eXtension), the generation of signal is based on a single fluorescent dye molecule that is attached to an oligonucleotide close to the 3′-end. A primer design software is available that identifies LUX primer pairs based on a set of rules for optimum signal development. The use of LUX fluorogenic primers to determine the expression patterns of various transcripts during differentiation in the P-19 mouse neuronal model is described.
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