• 我要登录|
  • 免费注册
    |
  • 我的丁香通
    • 企业机构:
    • 成为企业机构
    • 个人用户:
    • 个人中心
  • 移动端
    移动端
丁香通 logo丁香实验_LOGO
搜实验

    大家都在搜

      大家都在搜

        0 人通过求购买到了急需的产品
        免费发布求购
        发布求购
        点赞
        收藏
        wx-share
        分享

        Methods of Rotavirus Detection, Sero- and Genotyping, Sequencing, and Phylogenetic Analysis

        互联网

        449
        The clinical symptoms associated with rotavirus (RV) gastroenteritis are not sufficiently characteristic to distinguish between RV infection and other causes of gastroenteritis. Therefore, laboratory procedures, including electron microscopy (EM) (1 ), enzyme-linked immunosorbent assays (ELISA) (2 ), passive particle agglutination tests (PPAT) (3 ,4 ), polyacrylamide gel electrophoresis (PAGE) (5 ), or reverse transcription-polymerase chain reaction (RT-PCR) (6 8 ), are necessary to confirm a clinical diagnosis of RV gastroenteritis. Virus-shedding in the feces of RV-infected individuals, with up to 1011 virus particles/mL feces at the peak of diarrhea, coincides with the duration of the diarrhea. However, virus-shedding can continue once symptoms have ceased (9 ). Laboratory diagnosis is relatively easy, either through the visualization of virus particles by EM, the detection of virus antigen (Ag) by ELISA or PPAT, or the detection of the virus genome by PAGE or RT-PCR.
        ad image
        提问
        扫一扫
        丁香实验小程序二维码
        实验小助手
        丁香实验公众号二维码
        扫码领资料
        反馈
        TOP
        打开小程序