• 我要登录|
  • 免费注册
    |
  • 我的丁香通
    • 企业机构:
    • 成为企业机构
    • 个人用户:
    • 个人中心
  • 移动端
    移动端
丁香通 logo丁香实验_LOGO
搜实验

    大家都在搜

      大家都在搜

        0 人通过求购买到了急需的产品
        免费发布求购
        发布求购
        点赞
        收藏
        wx-share
        分享

        Live Cell Calcium Imaging of Dissociated Vomeronasal Neurons

        互联网

        487
        Sensory neurons in the vomeronasal organ (VNO) are thought to mediate a specialized olfactory response. Currently, very little is known about the identity of stimulating ligands or their cognate receptors that initiate neural activation. Each sensory neuron is thought to express 1 of approximately 250 variants of Vmn1Rs, Vmn2Rs (A, B, or D), or FPRs which enables it to be tuned to a subset of ligands (Touhara and Vosshall, Annu Rev Physiol 71:307–332, 2009). The logic of how different sources of native odors or purified ligands are detected by this complex sensory repertoire remains mostly unknown. Here, we describe a method to compare and analyze the response of VNO sensory neurons to multiple stimuli using conventional calcium imaging. This method differs from other olfactory imaging approaches in that we dissociate the tightly packed sensory epithelium into individual single cells. The advantages of this approach include (1) the use of a relatively simple approach and inexpensive microscopy, (2) comparative analysis of several hundreds of neurons to multiple stimuli with single-cell resolution, and (3) the possibility of isolating single cells of interest to further analyze by molecular biology techniques including in situ RNA hybridization, immunofluorescence, or creating single-cell cDNA libraries (Malnic et al., Cell 96:713–723, 1999).
        ad image
        提问
        扫一扫
        丁香实验小程序二维码
        实验小助手
        丁香实验公众号二维码
        扫码领资料
        反馈
        TOP
        打开小程序