Measurement of Aldehyde Formation as an Assay for Polyamine Oxidase Activity
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The polyamines spermine and spermidine can be metabolized by polyamine oxidases in two ways, oxidative determination or oxidative cleavage ( 1 , 2 ) ( see Chapter 1 ). An example of the former is the amine oxidase present in bovine (and other ruminant) sera ( 3 ) that deaminates polyamines in accordance with Fig. 1. In contrast, tissue polyamine oxidase (typified by rat liver polyamine oxidase) ( 4 ) cleaves spermine or spermidine with the release of an aminopropyl moiety (Fig. 2 ).
Fig. 1. Oxidation of spermine or spermidine by bovine serum amine oxidase.
Fig. 2. Oxidation of spermine or spermidine by rat liver polyamine oxidase.