Extraction and Detection of Arabinogalactan Proteins

Arabinogalactan proteins are a diverse group of plant cell wall-associated proteoglycans. While structural and molecular genetic analyses have contributed to the emerging improved understanding of the wide-range of biological processes in which AGPs are implicated; the ability to detect, localise, and quantify them is fundamentally important. This chapter describes two commonly used methods, histological staining and radial gel diffusion, both of which utilise the ability of Yariv reagent to bind to AGPs.