• 我要登录|
  • 免费注册
    |
  • 我的丁香通
    • 企业机构:
    • 成为企业机构
    • 个人用户:
    • 个人中心
  • 移动端
    移动端
丁香通 logo丁香实验_LOGO
搜实验

    大家都在搜

      大家都在搜

        0 人通过求购买到了急需的产品
        免费发布求购
        发布求购
        点赞
        收藏
        wx-share
        分享

        The SFV Gene Expression System

        互联网

        584
        The SFV Expression System is a DNA expression system used to produce recombinant protein in eukaryotic cells (1 ,2 ). The SFV system is based on the Semliki Forest Vnus (SFV), which has several features that provide distinct advantages for a good cDNA expression system. These are:
        1. 
        The SFV RNA genome has a positive polarity and thus functions directly as an mRNA. Infectious RNA molecules can be therefore obtained by transcription from a full-length cDNA copy of the genome,
        2. 
        The SFV RNA molecule codes for its own RNA rephcase. Consequently, within a few hours after infection, up to 200,000 copies of the plus-RNAs are made in a single cell (3 ),
        SFV replication occurs in the cell cytoplasm, where the virus replicase transcribes and caps the subgenomes for production of the structural proteins (4 ) This eliminates problems, such as mRNA splicing, limitations in transcription factors, problems with capping effctency, and mRNA transport,
        4. 
        The cytopathic effects in infected cells appear late during infection. Thus, there is an extensive time window of approx 4–24 h after infection during which a very high expression level of the SFV structural proteins is obtained without significant morphologrcal change of host cells, and
        5. 
        Under laboratory conditions, SFV infects a broad range of different cultured cells mammalian, avian, reptilian, amphibian, and insect cells.
        ad image
        提问
        扫一扫
        丁香实验小程序二维码
        实验小助手
        丁香实验公众号二维码
        扫码领资料
        反馈
        TOP
        打开小程序