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        Nanoscale Investigation on E. coli Adhesion to Modified Silicone Surfaces

        互联网

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        Bacterial infection is a major challenge in biomaterials development. The adhesion of microorganisms to the material surface is the first step in infectious conditions and this quickly leads to the formation of biofilms on a material surface. A unique attribute of atomic force microscopy (AFM) is that it reveals not only the morphology of cells and the surface roughness of the substrate, but it can also quantify the adhesion force between bacteria and surfaces. We have shown that fluoroalkylsilane (FAS) and octadecyltrichlorosilane (OTS)-coated silicone samples exhibit greater potential for reducing E. coli JM 109 adhesion than heparin- and hyaluronan-modified samples. The force curves obtained from AFM can be used as a primary indicator in predicting bacterial adhesion.
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