Site-Specific Incorporation of Unnatural Amino Acids as Probes for Protein Conformational Changes
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Site-specific in vivo incorporation of unnatural amino acids provides powerful tools for the study of protein interaction and dynamics. Here, we provide a protocol for the incorporation of six such UAA probes into a GFP reporter system, expressed in Escherichia coli from both arabinose and lactose-inducible expression plasmids using an autoinduction media.
![Phenol, 2,2′-[(1R,2R)-1,2-cyclohexanediylbis[(E)-nitrilomethylidyne]]bis[4-(1,1-dimethylethyl)-6-methyl-4-tert-butyl-2-[(E)-{[(1R,2R)-2-[(E)-[(5-tert-butyl-2-hydroxy-3-methylphenyl)methylidene]amino]cyclohexyl]imino}methyl]-6-methylphenol，1095419-61-6，阿拉丁](https://img1.dxycdn.com/p/s14/2024/0619/963/4625746064924733081.jpg!wh200)
