细胞遗传学――比较基因组杂交(CGH)

・         Comparative Genomic Hybridization ( CGH) CGH is a molecular Cytogenetic method of screening a tumor for genetic changes. The alterations are classified as DNA gains and losses and reveal a characteristic pattern that includes mutations at chromosomal and subchromosomal levels. This protocol gives detailed procedures on CGH as follows:
Metaphase chromosome preparation
DNA preparation by cryostom tissue dissection
DNA labeling by nick translation
Hybridization
DNA detection
Image capture
Image analysis
DAPI chromosome identification
  

・         Comparative Genomic Hybridization (CGH)
Detailed protocol for CGH, including slide and probe preparation, detection and staining
  

• Direct CGH (Waldman Lab)
    
• Indirect CGH (Waldman Lab)
    
• Nick Translation of DNA for CGH (Waldman Lab)